NiCoP/g-C[sub.3]N[sub.4] Nanocomposites-Based Electrochemical Immunosensor for Sensitive Detection of Procalcitonin

• Published in: Sensors (Basel, Switzerland) Switzerland), 2023-04, Vol.23 (9)
• Main Authors: Chen, Furong, Bao, Layue, Zhang, Ying, Wang, Ruili, Liu, Jinghai, Hai, Wenfeng, Liu, Yushuang
• Format: Article
• Language: English
• Subjects: Bacterial infections; Electric properties; Medicine; Practice; Sensors
• ISSN: 1424-8220; 1424-8220
• DOI: 10.3390/s23094348

Herein, an ultra-sensitive and facile electrochemical biosensor for procalcitonin (PCT) detection was developed based on NiCoP/g-C[sub.3]N[sub.4] nanocomposites. Firstly, NiCoP/g-C[sub.3]N[sub.4] nanocomposites were synthesized using hydrothermal methods and then functionalized on the electrode surface by π-π stacking. Afterward, the monoclonal antibody that can specifically capture the PCT was successfully linked onto the surface of the nanocomposites with a 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-Hydroxysuccinimide (NHS) condensation reaction. Finally, the modified sensor was employed for the electrochemical analysis of PCT using differential Pulse Voltammetry(DPV). Notably, the larger surface area of g-C[sub.3]N[sub.4] and the higher electron transfer capacity of NiCoP/g-C[sub.3]N[sub.4] endow this sensor with a wider detection range (1 ag/mL to 10 ng/mL) and an ultra-low limit of detection (0.6 ag/mL, S/N = 3). In addition, this strategy was also successfully applied to the detection of PCT in the diluted human serum sample, demonstrating that the developed immunosensors have the potential for application in clinical testing.