Construction and Comprehensive Analysis of miRNAs and Target mRNAs in ILongissimus dorsi/I Muscle of Queshan Black and Large White Pigs

In this study, the miRNA-mRNA combination analysis of the longissimus dorsi of adult Queshan Black and Large White pigs was carried out by RNA-seq technology, and the molecular mechanism affecting pork quality traits was revealed. The results showed that 39 miRNAs were differentially expressed betwe...

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Bibliographic Details
Published in:Life (Basel, Switzerland) Switzerland), 2022-11, Vol.12 (11)
Main Authors: Han, Xuelei, Qi, Kunlong, Song, Chenglei, Dou, Yaqing, Liu, Yingke, Li, Chenlei, Wei, Yilin, Qiao, Ruimin, Li, Xiuling, Yang, Feng, Wang, Kejun, Li, Xinjian
Format: Article
Language:English
Subjects:
Analysis
Cell differentiation
Genes
Genetic aspects
Messenger RNA
Methylation
MicroRNA
Muscles
Pork
Pork industry
Quality management
Swine
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Summary:In this study, the miRNA-mRNA combination analysis of the longissimus dorsi of adult Queshan Black and Large White pigs was carried out by RNA-seq technology, and the molecular mechanism affecting pork quality traits was revealed. The results showed that 39 miRNAs were differentially expressed between Queshan Black and Large White pigs. A functional enrichment analysis of their target genes showed that they were mainly enriched in pathways related to adipogenesis and cell proliferation and differentiation. In addition, miR-328, as a key miRNA, can affect the proliferation of 3T3-L1 cells. This study revealed the miRNA-mRNA regulatory axis affecting fat deposition and skeletal muscle mass and provided a theoretical basis for further study of the molecular regulation mechanism of meat quality traits.
ISSN:2075-1729
2075-1729
DOI:10.3390/life12111814