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Study on Anti-Constipation Effects of IHemerocallis citrina/I Baroni through a Novel Strategy of Network Pharmacology Screening

Daylily (Hemerocallis citrina Baroni) is an edible plant widely distributed worldwide, especially in Asia. It has traditionally been considered a potential anti-constipation vegetable. This study aimed to investigate the anti-constipation effects of daylily from the perspective of gastro-intestinal...

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Published in:International journal of molecular sciences 2023-03, Vol.24 (5)
Main Authors: Liang, Yuxuan, Wei, Xiaoyi, Ren, Rui, Zhang, Xuebin, Tang, Xiyao, Yang, Jinglan, Wei, Xiaoqun, Huang, Riming, Hardiman, Gary, Sun, Yuanming, Wang, Hong
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container_title International journal of molecular sciences
container_volume 24
creator Liang, Yuxuan
Wei, Xiaoyi
Ren, Rui
Zhang, Xuebin
Tang, Xiyao
Yang, Jinglan
Wei, Xiaoqun
Huang, Riming
Hardiman, Gary
Sun, Yuanming
Wang, Hong
description Daylily (Hemerocallis citrina Baroni) is an edible plant widely distributed worldwide, especially in Asia. It has traditionally been considered a potential anti-constipation vegetable. This study aimed to investigate the anti-constipation effects of daylily from the perspective of gastro-intestinal transit, defecation parameters, short-chain organic acids, gut microbiome, transcriptomes and network pharmacology. The results show that dried daylily (DHC) intake accelerated the defecation frequency of mice, while it did not significantly alter the levels of short-chain organic acids in the cecum. The 16S rRNA sequencing showed that DHC elevated the abundance of Akkermansia, Bifidobacterium and Flavonifractor, while it reduced the level of pathogens (such as Helicobacter and Vibrio). Furthermore, a transcriptomics analysis revealed 736 differentially expressed genes (DEGs) after DHC treatment, which are mainly enriched in the olfactory transduction pathway. The integration of transcriptomes and network pharmacology revealed seven overlapping targets (Alb, Drd2, Igf2, Pon1, Tshr, Mc2r and Nalcn). A qPCR analysis further showed that DHC reduced the expression of Alb, Pon1 and Cnr1 in the colon of constipated mice. Our findings provide a novel insight into the anti-constipation effects of DHC.
doi_str_mv 10.3390/ijms24054844
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subjects Amino acids
Constipation
Dopamine receptors
Pharmacology
RNA
title Study on Anti-Constipation Effects of IHemerocallis citrina/I Baroni through a Novel Strategy of Network Pharmacology Screening
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