Exploring the Role of a Novel Interleukin-17 Homolog from Invertebrate Marine Mussel IMytilus coruscus/I in Innate Immune Response: Is Negative Regulation by IMc/I-Novel_miR_145 the Key?

Interleukin-17 (IL-17) represents a class of proinflammatory cytokines involved in chronic inflammatory and degenerative disorders. Prior to this study, it was predicted that an IL-17 homolog could be targeted by Mc-novel_miR_145 to participate in the immune response of Mytilus coruscus. This study...

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Bibliographic Details
Published in:International journal of molecular sciences 2023-03, Vol.24 (6)
Main Authors: Chen, Xinglu, Qiu, Longmei, Si, Xirui, Zhang, Xiaolin, Guo, Baoying, Liao, Zhi, Yan, Xiaojun, Qi, Pengzhi
Format: Article
Language:English
Subjects:
Analysis
Antisense RNA
Cells
Immune response
Interleukins
Medical research
Medicine, Experimental
Molecular biology
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Summary:Interleukin-17 (IL-17) represents a class of proinflammatory cytokines involved in chronic inflammatory and degenerative disorders. Prior to this study, it was predicted that an IL-17 homolog could be targeted by Mc-novel_miR_145 to participate in the immune response of Mytilus coruscus. This study employed a variety of molecular and cell biology research methods to explore the association between Mc-novel_miR_145 and IL-17 homolog and their immunomodulatory effects. The bioinformatics prediction confirmed the affiliation of the IL-17 homolog with the mussel IL-17 family, followed by quantitative real-time PCR assays (qPCR) to demonstrate that McIL-17-3 was highly expressed in immune-associated tissues and responded to bacterial challenges. Results from luciferase reporter assays confirmed the potential of McIL-17-3 to activate downstream NF-κb and its targeting by Mc-novel_miR_145 in HEK293 cells. The study also produced McIL-17-3 antiserum and found that Mc-novel_miR_145 negatively regulates McIL-17-3 via western blotting and qPCR assays. Furthermore, flow cytometry analysis indicated that Mc-novel_miR_145 negatively regulated McIL-17-3 to alleviate LPS-induced apoptosis. Collectively, the current results showed that McIL-17-3 played an important role in molluscan immune defense against bacterial attack. Furthermore, McIL-17-3 was negatively regulated by Mc-novel_miR_145 to participate in LPS-induced apoptosis. Our findings provide new insights into noncoding RNA regulation in invertebrate models.
ISSN:1422-0067
DOI:10.3390/ijms24065928