DoMYB5 and DobHLH24, Transcription Factors Involved in Regulating Anthocyanin Accumulation in IDendrobium officinale/I

As a kind of orchid plant with both medicinal and ornamental value, Dendrobium officinale has garnered increasing research attention in recent years. The MYB and bHLH transcription factors play important roles in the synthesis and accumulation of anthocyanin. However, how MYB and bHLH transcription...

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Bibliographic Details
Published in:International journal of molecular sciences 2023-04, Vol.24 (8)
Main Authors: Yang, Kun, Hou, Yibin, Wu, Mei, Pan, Qiuyu, Xie, Yilong, Zhang, Yusen, Sun, Fenghang, Zhang, Zhizhong, Wu, Jinghua
Format: Article
Language:English
Subjects:
Anthocyanin
DNA binding proteins
Flowers
Medicine, Botanic
Medicine, Herbal
Online Access:Get full text
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Summary:As a kind of orchid plant with both medicinal and ornamental value, Dendrobium officinale has garnered increasing research attention in recent years. The MYB and bHLH transcription factors play important roles in the synthesis and accumulation of anthocyanin. However, how MYB and bHLH transcription factors work in the synthesis and accumulation of anthocyanin in D. officinale is still unclear. In this study, we cloned and characterized one MYB and one bHLH transcription factor, namely, D. officinale MYB5 (DoMYB5) and D. officinaleb bHLH24 (DobHLH24), respectively. Their expression levels were positively correlated with the anthocyanin content in the flowers, stems, and leaves of D. officinale varieties with different colors. The transient expression of DoMYB5 and DobHLH24 in D. officinale leaf and their stable expression in tobacco significantly promoted the accumulation of anthocyanin. Both DoMYB5 and DobHLH24 could directly bind to the promoters of D. officinale CHS (DoCHS) and D. officinale DFR (DoDFR) and regulate DoCHS and DoDFR expression. The co-transformation of the two transcription factors significantly enhanced the expression levels of DoCHS and DoDFR. DoMYB5 and DobHLH24 may enhance the regulatory effect by forming heterodimers. Drawing on the results of our experiments, we propose that DobHLH24 may function as a regulatory partner by interacting directly with DoMYB5 to stimulate anthocyanin accumulation in D. officinale.
ISSN:1422-0067
DOI:10.3390/ijms24087552