IAbelmoschus eculentus/I Seed Extract Exhibits In Vitro and In Vivo Anti-Alzheimer’s Potential Supported by Metabolomic and Computational Investigation

Abelmoschus esculentus Linn. (okra, F. Malvaceae) is a fruit widely consumed all over the world. In our study, the anti-Alzheimer’s potential of A. esculentus was evaluated. An in vitro DPPH free radical assay on A. esculentus seed’s total extract and AChE inhibition potential screening indicated a...

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Bibliographic Details
Published in:Plants (Basel) 2023-06, Vol.12 (12)
Main Authors: Bakhsh, Hussain T, Mokhtar, Fatma A, Elmaidomy, Abeer H, Aly, Hanan F, Younis, Eman A, Alzubaidi, Mubarak A, Altemani, Faisal H, Algehainy, Naseh A, Majrashi, Mohammed Ali A, Alsenani, Faisal, Bringmann, Gerhard, Abdelmohsen, Usama Ramadan, Abdelhafez, Omnia Hesham
Format: Article
Language:English
Subjects:
Alzheimer's disease
Chemical properties
Exhibitions
Health aspects
Investigations
Materia medica, Vegetable
Okra
Plant extracts
Seeds
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Summary:Abelmoschus esculentus Linn. (okra, F. Malvaceae) is a fruit widely consumed all over the world. In our study, the anti-Alzheimer’s potential of A. esculentus was evaluated. An in vitro DPPH free radical assay on A. esculentus seed’s total extract and AChE inhibition potential screening indicated a significant anti-Alzheimer’s activity of the extract, which was confirmed through an in vivo study in an aluminum-intoxicated rat model. Additionally, in vivo results demonstrated significant improvement in Alzheimer’s rats, which was confirmed by improving T-maze, beam balance tests, lower serum levels of AChE, norepinephrine, glycated end products, IL-6, and MDA. The levels of dopamine, BDNF, GSH, and TAC returned to normal values during the study. Moreover, histological investigations of brain tissue revealed that the destruction in collagen fiber nearly returns back to the normal pattern. Metabolomic analysis of the ethanolic extract of A. esculentus seeds via LC–HR-ESI-MS dereplicated ten compounds. A network pharmacology study displayed the relation between identified compounds and 136 genes, among which 84 genes related to Alzheimer’s disorders, and focused on AChE, APP, BACE1, MAPT and TNF genes with interactions to all Alzheimer’s disorders. Consequently, the results revealed in our study grant potential dietary elements for the management of Alzheimer’s disorders.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants12122382