Lauroylated, Acetylated, and Succinylated IAgave tequilana/I Fructans Fractions: Structural Characterization, Prebiotic, Antibacterial Activity and Their Effect on ILactobacillus paracasei/I under Gastrointestinal Conditions

• Published in: Polymers 2023-07, Vol.15 (14)
• Main Authors: Díaz-Ramos, Dafne I, O, García-Barradas, Oscar, Chacón-López, Ma, Montalvo-González, Efigenia, Pascual-Pineda, Luz A, Valenzuela-Vázquez, Uri, Jiménez-Fernández, Maribel
• Format: Article
• Language: English
• Subjects: Antibacterial agents; Escherichia coli; Gastrointestinal system; Polymerization; Scientific equipment and supplies industry
• ISSN: 2073-4360; 2073-4360
• DOI: 10.3390/polym15143115

The effect of chemical modification of fractions of native agave fructans (NAF), high performance (HPAF), and a high degree of polymerization (HDPAF) by lauroylation, acetylation, and succinylation reactions on their prebiotic activity, antibacterial properties were evaluated and survival of L. paracasei in a simulated gastrointestinal system. The characterization of the reactions was confirmed by NMR and FTIR. The lauroylated and succinylated fructan fractions showed higher antibacterial activity against pathogenic bacteria such as Escherichia coli, Enterococcus faecalis and Staphylococcus aureus than the unmodified ones. Analyses with L. paracasei showed that the acetylated fructan fractions had a greater prebiotic effect, and simulated gastrointestinal tests demonstrated that the acetylated and succinylated fractions favored the survival of L. paracasei during the gastrointestinal phase. The effect of modifying the agave fructans fractions on the evaluated properties depended on the structure, size, and polarity of each incorporated functional group, as well as the degree of polymerization and substitution of each fraction. These results show that the chemical modification of the fructan fractions analyzed improves their functional properties, offering an alternative in the food and pharmaceutical industry.