Expression, activity, and consequences of biochemical inhibition of [alpha]- and [beta]-glucosidases in different life stages of Culex quinquefasciatus

• Published in: PloS one 2023-08, Vol.18 (8), p.e0286609
• Main Authors: Burgess, Edwin R, Sanscrainte, Neil D, Taylor, Caitlin E, Buss, Lyle J, Estep, Alden S
• Format: Article
• Language: English
• Subjects: Analysis; Biological control; Environmental aspects; Enzymes; Gene expression; Genetic aspects; Glycogen; Mosquitoes; Pests
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0286609

Mosquitoes have a wide range of digestive enzymes that enable them to utilize requisite blood and sugar meals for survival and reproduction. Sugar meals, typically derived from plant sources, are critical to maintain energy in both male and female mosquitoes, whereas blood meals are taken only by females to complete oogenesis. Enzymes involved in sugar digestion have been the subject of study for decades but have been limited to a relatively narrow range of mosquito species. The southern house mosquito, Culex quinquefasciatus, is of public health importance and seldom considered in these types of studies outside of topics related to Bacillus sphaericus, a biocontrol agent that requires interaction with a specific gut-associated [alpha]-glucosidase. Here we sought to describe the nature of [alpha]-glucosidases and unexplored [beta]-glucosidases that may aid Cx. quinquefasciatus larvae in acquiring nutrients from cellulosic sources in their aquatic habitats. Consistent with our hypothesis, we found both [alpha]- and [beta]-glucosidase activity in larvae. Interestingly, [beta]-glucosidase activity all but disappeared at the pupal stage and remained low in adults, while [alpha]-glucosidase activity remained in the pupal stage and then exceeded larval activity by approximately 1.5-fold. The expression patterns of the putative [alpha]- and [beta]-glucosidase genes chosen did not consistently align with observed enzyme activities. When the [alpha]-glucosidase inhibitor acarbose was administered to adults, mortality was seen especially in males but also in females after two days of exposure and key energetic storage molecules, glycogen and lipids, were significantly lower than controls. In contrast, administering the [beta]-glucosidase inhibitor conduritol [beta]-epoxide to larvae did not produce mortality even at the highest soluble concentration. Here we provide insights into the importance of [alpha]- and [beta]-glucosidases on the survival of Cx. quinquefasciatus in their three mobile life stages.