Mechanism underlying the effect of MnO.sub.2 nanosheets for A549 cell chemodynamic therapy

MnO.sub.2 nanosheets (MnO.sub.2NSs) were synthesized by one-step method, and MnO.sub.2NSs were applied to A549 cell chemodynamic Therapy (CDT). The cytotoxicity, redox ability, and reactive oxygen species production of MnO.sub.2NSs have been investigated, and differences in cell metabolism during CD...

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Bibliographic Details
Published in:Mikrochimica acta (1966) 2023-10, Vol.190 (10)
Main Authors: Liu, Jian, Wen, Changchun, Hu, Miaomiao, Leng, Nan, Lin, Xiang-Cheng
Format: Article
Language:English
Subjects:
Development and progression
Health aspects
Liquid chromatography
Lung cancer
Mass spectrometry
Metabolites
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Summary:MnO.sub.2 nanosheets (MnO.sub.2NSs) were synthesized by one-step method, and MnO.sub.2NSs were applied to A549 cell chemodynamic Therapy (CDT). The cytotoxicity, redox ability, and reactive oxygen species production of MnO.sub.2NSs have been investigated, and differences in cell metabolism during CDT were determined using liquid chromatography-mass spectrometry (LC-MS/MS). In addition, the metabolites of A549 lung cancer cells affected by MnO.sub.2NSs treatment are identified; metabolite differences were identified by PCA, PLS-DA, orthogonal PLS-DA, and other methods; and these differences were analyzed using non-targeted metabolomics. We found that A549 cells which were treated by MnO.sub.2NSs have 17 different metabolites and 9 metabolic pathways that varied markedly. Owing to their unique composition, structure, and physicochemical properties, MnO.sub.2NSs and their composites have become a favored type of nanomaterial used for CDT in cancer therapy. This work provides insights into the mechanism underlying the effects of MnO.sub.2NSs on the tumor microenvironment of A549 lung cancer cells, effectively making up for the deficiency of the study on cellular mechanism of CDT-induced apoptosis of cancer cells. It could aid the development of cancer CDT treatment strategies and help improve the use of nanomaterials in the clinical field. Graphical
ISSN:0026-3672
DOI:10.1007/s00604-023-05974-x