MucR from ISinorhizobium meliloti/I: New Insights into Its DNA Targets and Its Ability to Oligomerize

Proteins of the MucR/Ros family play a crucial role in bacterial infection or symbiosis with eukaryotic hosts. MucR from Sinorhizobium meliloti plays a regulatory role in establishing symbiosis with the host plant, both dependent and independent of Quorum Sensing. Here, we report the first character...

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Bibliographic Details
Published in:International journal of molecular sciences 2023-09, Vol.24 (19)
Main Authors: Slapakova, Ma, Sgambati, Domenico, Pirone, Luciano, Russo, Veronica, D’Abrosca, Gianluca, Valletta, Mariangela, Russo, Rosita, Chambery, Angela, Malgieri, Gaetano, Pedone, Emilia Maria, Dame, Remus Thei, Pedone, Paolo Vincenzo, Baglivo, Ilaria
Format: Article
Language:English
Subjects:
Bacterial infections
DNA
DNA-ligand interactions
Genes
Genetic aspects
Genetic research
Health aspects
International economic relations
Mass spectrometry
Oligomers
Proteins
Scientific equipment and supplies industry
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Summary:Proteins of the MucR/Ros family play a crucial role in bacterial infection or symbiosis with eukaryotic hosts. MucR from Sinorhizobium meliloti plays a regulatory role in establishing symbiosis with the host plant, both dependent and independent of Quorum Sensing. Here, we report the first characterization of MucR isolated from Sinorhizobium meliloti by mass spectrometry and demonstrate that this protein forms higher-order oligomers in its native condition of expression by SEC-MALS. We show that MucR purified from Sinorhizobium meliloti can bind DNA and recognize the region upstream of the ndvA gene in EMSA, revealing that this gene is a direct target of MucR. Although MucR DNA binding activity was already described, a detailed characterization of Sinorhizobium meliloti DNA targets has never been reported. We, thus, analyze sequences recognized by MucR in the rem gene promoter, showing that this protein recognizes AT-rich sequences and does not require a consensus sequence to bind DNA. Furthermore, we investigate the dependence of MucR DNA binding on the length of DNA targets. Taken together, our studies establish MucR from Sinorhizobium meliloti as a member of a new family of Histone-like Nucleoid Structuring (H-NS) proteins, thus explaining the multifaceted role of this protein in many species of alpha-proteobacteria.
ISSN:1422-0067
DOI:10.3390/ijms241914702