Coordination of m[sup.6]A mRNA Methylation and Gene Transcriptome in Sugarcane Response to Drought Stress

The N[sup.6] -methyladenosine (m[sup.6] A) methylation of mRNA is involved in biological processes essential for plant growth. To explore the m[sup.6] A modification of sugarcane and reveal its regulatory function, methylated RNA immunoprecipitation sequencing (MeRIP-seq) was used to construct the m...

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Published in:Plants (Basel) 2023-10, Vol.12 (21)
Main Authors: Wei, Jinju, Li, Haibi, Gui, Yiyun, Zhou, Hui, Zhang, Ronghua, Zhu, Kai, Liu, Xihui
Format: Article
Language:English
Subjects:
Arabidopsis thaliana
Botanical research
China
Droughts
Environmental aspects
Epigenetic inheritance
Evolution
Genetic aspects
Messenger RNA
Methylation
Methyltransferases
MicroRNA
Photosynthesis
Physiological aspects
Plant genetics
Plants
Resveratrol
Rice
Stress (Physiology)
Sugarcane
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Summary:The N[sup.6] -methyladenosine (m[sup.6] A) methylation of mRNA is involved in biological processes essential for plant growth. To explore the m[sup.6] A modification of sugarcane and reveal its regulatory function, methylated RNA immunoprecipitation sequencing (MeRIP-seq) was used to construct the m[sup.6] A map of sugarcane. In this study, m[sup.6] A sites of sugarcane transcriptome were significantly enriched around the stop codon and within 3′-untranslated regions (3′UTR). Gene ontology (GO) analysis showed that the m[sup.6] A modification genes are associated with metabolic biosynthesis. In addition, the m[sup.6] A modification of drought-resistant transcript mRNA increased significantly under drought (DR) treatment, resulting in enhanced mRNA stability, which is involved in regulating sugarcane drought resistance. GO and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment results showed that differentially methylated peak (DMP) modification of differentially expressed genes (DEGs) in DR were particularly associated with abscisic acid (ABA) biosynthesis. The upregulated genes were significantly enriched in the ABA metabolism, ethylene response, fatty acid metabolism, and negative regulation of the abscisic acid activation signaling pathway. These findings provide a basis and resource for sugarcane RNA epigenetic studies and further increase our knowledge of the functions of m[sup.6] A modifications in RNA under abiotic stress.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants12213668