Responsiveness of Candidate Genes on ICoPv01CDRK/PhgPv01CDRK/I Loci in Common Bean Challenged by Anthracnose and Angular Leaf Spot Pathogens

Anthracnose (ANT) and angular leaf spot (ALS) are significant diseases in common bean, leading to considerable yield losses under specific environmental conditions. The California Dark Red Kidney (CDRK) bean cultivar is known for its resistance to multiple races of both pathogens. Previous studies h...

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Bibliographic Details
Published in:International journal of molecular sciences 2023-11, Vol.24 (22)
Main Authors: Lovatto, Maike, Gonçalves-Vidigal, Maria Celeste, Vaz Bisneta, Mariana, Calvi, Alexandre Catto, Mazucheli, Josmar, Vidigal Filho, Pedro Soares, Miranda, Eduardo Gomes Rosa, Melotto, Maeli
Format: Article
Language:English
Subjects:
Analysis
Defense industry
Development and progression
Drug resistance in microorganisms
Gene expression
Genetic research
Pathogenic microorganisms
Plant genetics
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Summary:Anthracnose (ANT) and angular leaf spot (ALS) are significant diseases in common bean, leading to considerable yield losses under specific environmental conditions. The California Dark Red Kidney (CDRK) bean cultivar is known for its resistance to multiple races of both pathogens. Previous studies have identified the CoPv01CDRK/PhgPv01CDRK resistance loci on chromosome Pv01. Here, we evaluated the expression levels of ten candidate genes near the CoPv01CDRK/PhgPv01CDRK loci and plant defense genes using quantitative real-time PCR in CDRK cultivar inoculated with races 73 of Colletotrichum lindemuthianum and 63-39 of Pseudocercospora griseola. Gene expression analysis revealed that the Phvul.001G246300 gene exhibited the most elevated levels, showing remarkable 7.8-fold and 8.5-fold increases for ANT and ALS, respectively. The Phvul.001G246300 gene encodes an abscisic acid (ABA) receptor with pyrabactin resistance, PYR1-like (PYL) protein, which plays a central role in the crosstalk between ABA and jasmonic acid responses. Interestingly, our results also showed that the other defense genes were initially activated. These findings provide critical insights into the molecular mechanisms underlying plant defense against these diseases and could contribute to the development of more effective disease management strategies in the future.
ISSN:1422-0067
DOI:10.3390/ijms242216023