BINDING MECHANISM OF METHYLENE BLUE WITH SINGLE-STRANDED SYNTHETIC POLYNUCLEOTIDES poly

The binding of the thiazine dye methylene blue (MB) with single-stranded (ss-) polyribonucleotides poly(rA) and poly(rU) was studied using absorption spectroscopy and UV-melting. The binding mechanisms of MB to poly(rA) and poly(rU) were found to differ significantly. The obtained data indicated tha...

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Bibliographic Details
Published in:Journal of applied spectroscopy 2023-09, Vol.90 (4), p.742
Main Authors: Movsesyan, Z.O, Antonyan, A.P, Vardevanyan, P.O
Format: Article
Language:English
Subjects:
Ethylenediaminetetraacetic acid
Health aspects
Methylene blue
Molecular biology
RNA
Online Access:Get full text
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Summary:The binding of the thiazine dye methylene blue (MB) with single-stranded (ss-) polyribonucleotides poly(rA) and poly(rU) was studied using absorption spectroscopy and UV-melting. The binding mechanisms of MB to poly(rA) and poly(rU) were found to differ significantly. The obtained data indicated that the main mode of interaction between MB and ss-poly(rA) and ss-poly(rU) corresponded to semi-intercalation to electrostatic binding, respectively. MB was also found to contribute to the formation of the hybrid duplex poly(rA)-poly(rU).
ISSN:0021-9037
DOI:10.1007/s10812-023-01590-y