Gastrorepair potential of functional fermented orange beverage against ethanol-induced gastric ulcer in rats /Avaliação do potencial gastroreparador da bebida funcional fermentada de laranja contra úlcera gástrica induzida por etanol em ratos

• Published in: Ciência rural 2024-05, Vol.54 (6), p.1
• Main Authors: Mascarin, Laura Gizele, Franco, Fernanda Wouters, Reginato, Fernanda Ziegler, Graiczik, James Ramires Penteado, Pereira, William Nieckel, da Silva, João Osmar Fruet, Boldori, Jean Ramos, Aramburú, Jaime Sardá, Jr, da Costa, Silvio Teixeira, Denardin, Cristiane Casagrande, de Freitas Bauermann, Liliane, Somacal, Sabrina, Sautter, Cláudia Kaehler
• Format: Article
• Language: English
• ISSN: 0103-8478
• DOI: 10.1590/0103-8478cr20230162

Previous investigations have revealed that a functional fermented orange beverage presented in its composition different phenolic compounds, which through in silico investigation demonstrated to have biological effects of therapeutic importance as antioxidant, gastrorepair, and anti-ulcerative properties. Thus, this study confirmed in vivo, through a model of EtOH-induced gastric ulcers in rats, the beneficial properties indicated by the in silico tests. Gastric ulcer was induced by EtOH (intragastric) and was treated after 1 h with fermented orange beverage with and without Mentha piperita extract (0.5 mL/100 g w.b). Omeprazole was used as positive control. Histopathological evaluation revealed that EtOH administration resulted in the formation of gastric ulcers due to the reduction of the mucus layer, presence of hemorrhage, and infiltration of neutrophils in the stomach tissue of rats, and only treatment with omeprazole was able to reverse these changes. Additionally, EtOH administration altered the gastric juice volume and induced oxidative stress in the gastric tissue observed through the increase in lipid peroxidation (TBARS), reduction in the levels of non-protein thiols (NPSH), and alteration in the superoxide dismutase (SOD) activity. The ingestion of the fermented orange beverage increased NPSH levels and reduced changes in TBARS levels induced by ethanol. These findings suggested that the fermented orange beverage has antioxidant effects, as pointed out by in silico studies, but not gastrorepair and anti-ulcerative effects. Key words: gastric ulcer, oxidative stress, antioxidants defenses, polyphenols, functional beverages. Investigações anteriores revelaram que uma bebida funcional fermentada de laranja apresentou em sua composição diferentes compostos fenólicos, que através de investigações in silico demonstraram ter efeitos biológicos de importância terapêutica como propriedades antioxidantes, gastroreparadoras e antiulcerativas. Assim, o objetivo deste estudo foi confirmar in vivo, através de um modelo de úlcera gástrica induzida por EtOH em ratos, as propriedades benéficas indicadas pelos ensaios in silico. A úlcera gástrica foi induzida por EtOH (intragástrico) e tratada após 1 h com bebida fermentada de laranja com e sem o extrato de Mentha piperita (0,5 mL/100 g peso corporal). Omeprazol foi usado como controle positivo. A avaliação histopatológica revelou que a administração de EtOH resultou na formação de úlc