Fatty Oil of Descurainia Sophia Nanoparticles Improve Monocrotaline-Induced Pulmonary Hypertension in Rats Through PLC/IP3R/[Ca.sup.2+] Signaling Pathway

Purpose: Fatty oil of Descurainia Sophia (OIL) has poor stability and low solubility, which limits its pharmacological effects. We hypothesized that fatty oil nanoparticles (OIL-NPs) could overcome this limitation. The protective effect of OIL- NPs against monocrotaline-induced lung injury in rats w...

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Bibliographic Details
Published in:International journal of nanomedicine 2023-12, Vol.18, p.7483
Main Authors: Zheng, Yajuan, Yuan, Peipei, Zhang, Zhenkai, Fu, Yang, Li, Saifei, Ruan, Yuan, Li, Panying, Chen, Yi, Feng, Weisheng, Zheng, Xiaoke
Format: Article
Language:English
Subjects:
Immunohistochemistry
Inositol
Nanoparticles
Neomycin
Pulmonary hypertension
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Summary:Purpose: Fatty oil of Descurainia Sophia (OIL) has poor stability and low solubility, which limits its pharmacological effects. We hypothesized that fatty oil nanoparticles (OIL-NPs) could overcome this limitation. The protective effect of OIL- NPs against monocrotaline-induced lung injury in rats was studied. Methods: We prepared OIL-NPs by wrapping fatty oil with polylactic-polyglycolide nanoparticles (PLGA-NPs) and conducted in vivo and in vitro experiments to explore its anti-pulmonary hypertension (PH) effect. In vitro, we induced malignant proliferation of pulmonary artery smooth muscle cells (RPASMC) using anoxic chambers, and studied the effects of OIL-NPs on the malignant proliferation of RPASMC cells and phospholipase C (PLC)/inositol triphosphate receptor (IP3R)/[Ca.sup.2+] signal pathways. In vivo, we used small animal echocardiography, flow cytometry, immunohistochemistry, western blotting (WB), polymerase chain reaction (PCR) and metabolomics to explore the effects of OIL-NPs on the heart and lung pathological damage and PLC/IP3R/[Ca.sup.2+] signal pathway of pulmonary hypertension rats. Results: We prepared fatty into OIL-NPs. In vitro, OIL-NPs could improve the mitochondrial function and inhibit the malignant proliferation of RPASMC cells by inhibiting the PLC/IP3R/[Ca.sup.2+]signal pathway. In vivo, OIL-NPs could reduce the pulmonary artery pressure of rats and alleviate the pathological injury and inflammatory reaction of heart and lung by inhibiting the PLC/JP3R/[Ca.sup.2+] signal pathway. Conclusion: OIL-NPs have anti-pulmonary hypertension effect, and the mechanism may be related to the inhibition of PLC/IP3R/[Ca.sup.2+]signal pathway. Keywords: pulmonary hypertension, PLC/IP3R/[Ca.sup.2+], nanoparticles, RPASMC, fatty oil
ISSN:1178-2013
DOI:10.2147/IJN.S436866