Genetic Loci of Plant Pathogenic IDickeya solani/I IPO 2222 Expressed in Contact with Weed-Host Bittersweet Nightshade Plants

Dickeya solani, belonging to the Soft Rot Pectobacteriaceae, are aggressive necrotrophs, exhibiting both a wide geographic distribution and a wide host range that includes many angiosperm orders, both dicot and monocot plants, cultivated under all climatic conditions. Little is known about the infec...

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Bibliographic Details
Published in:International journal of molecular sciences 2024-02, Vol.25 (5)
Main Authors: Czajkowski, Robert, Krzyżanowska, Dorota M, Sokolova, Daryna, Rąbalski, Łukasz, Kosiński, Maciej, Jafra, Sylwia, Królicka, Aleksandra
Format: Article
Language:English
Subjects:
Amino acids
Gene mutations
Genetic research
Health aspects
Infection
Neomycin
Polysaccharides
Potatoes
Proteins
Transfer RNA
Virulence (Microbiology)
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Summary:Dickeya solani, belonging to the Soft Rot Pectobacteriaceae, are aggressive necrotrophs, exhibiting both a wide geographic distribution and a wide host range that includes many angiosperm orders, both dicot and monocot plants, cultivated under all climatic conditions. Little is known about the infection strategies D. solani employs to infect hosts other than potato (Solanum tuberosum L.). Our earlier study identified D. solani Tn5 mutants induced exclusively by the presence of the weed host S. dulcamara. The current study assessed the identity and virulence contribution of the selected genes mutated by the Tn5 insertions and induced by the presence of S. dulcamara. These genes encode proteins with functions linked to polyketide antibiotics and polysaccharide synthesis, membrane transport, stress response, and sugar and amino acid metabolism. Eight of these genes, encoding UvrY (GacA), tRNA guanosine transglycosylase Tgt, LPS-related WbeA, capsular biosynthesis protein VpsM, DltB alanine export protein, glycosyltransferase, putative transcription regulator YheO/PAS domain-containing protein, and a hypothetical protein, were required for virulence on S. dulcamara plants. The implications of D. solani interaction with a weed host, S. dulcamara, are discussed.
ISSN:1422-0067
DOI:10.3390/ijms25052794