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Profiling the Phytochemicals of IOrostachys margaritifolia/I: Biological Activities, LC-ESI/MS, and HPLC Analyses

Orostachys margaritifolia Y. N. Lee (OMY) is an endemic Korean plant in the family Crassulaceae that is known to contain a variety of bioactive compounds. To assess the physiological activities of an OMY ethanol extract, ABTS[sup.+] and DPPH radical scavenging assays and a nitric oxide (NO) inhibiti...

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Published in:Pharmaceuticals (Basel, Switzerland) Switzerland), 2024-02, Vol.17 (3)
Main Authors: Yoon, Nari, Yu, Hyeonjun, Tran, Gia Han, Ko, Chung Ho, Kim, Hoon, Yoon, Mi Jeong, Lee, Sanghyun
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container_title Pharmaceuticals (Basel, Switzerland)
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Yu, Hyeonjun
Tran, Gia Han
Ko, Chung Ho
Kim, Hoon
Yoon, Mi Jeong
Lee, Sanghyun
description Orostachys margaritifolia Y. N. Lee (OMY) is an endemic Korean plant in the family Crassulaceae that is known to contain a variety of bioactive compounds. To assess the physiological activities of an OMY ethanol extract, ABTS[sup.+] and DPPH radical scavenging assays and a nitric oxide (NO) inhibition assay were conducted. The phytochemical makeup of the extract was profiled via liquid chromatography-mass spectrometry (LC-ESI/MS) and high-performance liquid chromatography with a photodiode array detector (HPLC/PDA). The OMY extract was found to have weaker ABTS[sup.+] and DPPH radical scavenging activities than the control group (green tea). In the NO inhibition assay, the OMY extract induced a significant increase in macrophage cell viability but showed a lower NO inhibitory activity than l-NAME, producing an IC[sub.50] value of 202.6 μg/mL. The LC-ESI/MS and HPLC/PDA analyses identified isoquercitrin and astragalin in the OMY extract, quantifying their contents at 3.74 mg/g and 3.19 mg/g, respectively. The study revealed possibilities for the utilization of OMY as a future source of drugs for alleviating inflammation and diseases related to reactive oxygen species.
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subjects Anti-inflammatory drugs
Chemical properties
Crassulaceae
Health aspects
High performance liquid chromatography
Identification and classification
Mass spectrometry
Pharmaceutical research
Phytochemicals
title Profiling the Phytochemicals of IOrostachys margaritifolia/I: Biological Activities, LC-ESI/MS, and HPLC Analyses
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