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Profiling the Phytochemicals of IOrostachys margaritifolia/I: Biological Activities, LC-ESI/MS, and HPLC Analyses
Orostachys margaritifolia Y. N. Lee (OMY) is an endemic Korean plant in the family Crassulaceae that is known to contain a variety of bioactive compounds. To assess the physiological activities of an OMY ethanol extract, ABTS[sup.+] and DPPH radical scavenging assays and a nitric oxide (NO) inhibiti...
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Published in: | Pharmaceuticals (Basel, Switzerland) Switzerland), 2024-02, Vol.17 (3) |
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creator | Yoon, Nari Yu, Hyeonjun Tran, Gia Han Ko, Chung Ho Kim, Hoon Yoon, Mi Jeong Lee, Sanghyun |
description | Orostachys margaritifolia Y. N. Lee (OMY) is an endemic Korean plant in the family Crassulaceae that is known to contain a variety of bioactive compounds. To assess the physiological activities of an OMY ethanol extract, ABTS[sup.+] and DPPH radical scavenging assays and a nitric oxide (NO) inhibition assay were conducted. The phytochemical makeup of the extract was profiled via liquid chromatography-mass spectrometry (LC-ESI/MS) and high-performance liquid chromatography with a photodiode array detector (HPLC/PDA). The OMY extract was found to have weaker ABTS[sup.+] and DPPH radical scavenging activities than the control group (green tea). In the NO inhibition assay, the OMY extract induced a significant increase in macrophage cell viability but showed a lower NO inhibitory activity than l-NAME, producing an IC[sub.50] value of 202.6 μg/mL. The LC-ESI/MS and HPLC/PDA analyses identified isoquercitrin and astragalin in the OMY extract, quantifying their contents at 3.74 mg/g and 3.19 mg/g, respectively. The study revealed possibilities for the utilization of OMY as a future source of drugs for alleviating inflammation and diseases related to reactive oxygen species. |
doi_str_mv | 10.3390/ph17030290 |
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N. Lee (OMY) is an endemic Korean plant in the family Crassulaceae that is known to contain a variety of bioactive compounds. To assess the physiological activities of an OMY ethanol extract, ABTS[sup.+] and DPPH radical scavenging assays and a nitric oxide (NO) inhibition assay were conducted. The phytochemical makeup of the extract was profiled via liquid chromatography-mass spectrometry (LC-ESI/MS) and high-performance liquid chromatography with a photodiode array detector (HPLC/PDA). The OMY extract was found to have weaker ABTS[sup.+] and DPPH radical scavenging activities than the control group (green tea). In the NO inhibition assay, the OMY extract induced a significant increase in macrophage cell viability but showed a lower NO inhibitory activity than l-NAME, producing an IC[sub.50] value of 202.6 μg/mL. The LC-ESI/MS and HPLC/PDA analyses identified isoquercitrin and astragalin in the OMY extract, quantifying their contents at 3.74 mg/g and 3.19 mg/g, respectively. 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N. Lee (OMY) is an endemic Korean plant in the family Crassulaceae that is known to contain a variety of bioactive compounds. To assess the physiological activities of an OMY ethanol extract, ABTS[sup.+] and DPPH radical scavenging assays and a nitric oxide (NO) inhibition assay were conducted. The phytochemical makeup of the extract was profiled via liquid chromatography-mass spectrometry (LC-ESI/MS) and high-performance liquid chromatography with a photodiode array detector (HPLC/PDA). The OMY extract was found to have weaker ABTS[sup.+] and DPPH radical scavenging activities than the control group (green tea). In the NO inhibition assay, the OMY extract induced a significant increase in macrophage cell viability but showed a lower NO inhibitory activity than l-NAME, producing an IC[sub.50] value of 202.6 μg/mL. The LC-ESI/MS and HPLC/PDA analyses identified isoquercitrin and astragalin in the OMY extract, quantifying their contents at 3.74 mg/g and 3.19 mg/g, respectively. The study revealed possibilities for the utilization of OMY as a future source of drugs for alleviating inflammation and diseases related to reactive oxygen species.</description><subject>Anti-inflammatory drugs</subject><subject>Chemical properties</subject><subject>Crassulaceae</subject><subject>Health aspects</subject><subject>High performance liquid chromatography</subject><subject>Identification and classification</subject><subject>Mass spectrometry</subject><subject>Pharmaceutical research</subject><subject>Phytochemicals</subject><issn>1424-8247</issn><issn>1424-8247</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNptkE9Lw0AUxBdRsFYvfoIFr0379k-6G28xVBuItNDey2azm6ykWc0God_eFD30IHN4A_N7cxiEHgnMGUtg8dkQAQxoAldoQjjlkaRcXF_4W3QXwgdALAgnE_S17b11retqPDQGb5vT4HVjjk6rNmBvcb7pfRiUbk4BH1Vfq94NzvrWqUX-jF-cb319hnGqB_c9ZibMcJFFq12-eN_NsOoqvN4WGU471Z6CCffoxo7d5uHvTtH-dbXP1lGxecuztIjqpRCRNiKWZaIotVZSXWrghIOGkhC6BMFZyZQ0khGIScmWIk4qFicCrOGVVEDYFD391taqNQfXWT_0Sh9d0IdUSEljSqQYqfk_1KjqPIHvzLiNuXz4AXG8aPQ</recordid><startdate>20240201</startdate><enddate>20240201</enddate><creator>Yoon, Nari</creator><creator>Yu, Hyeonjun</creator><creator>Tran, Gia Han</creator><creator>Ko, Chung Ho</creator><creator>Kim, Hoon</creator><creator>Yoon, Mi Jeong</creator><creator>Lee, Sanghyun</creator><general>MDPI AG</general><scope/></search><sort><creationdate>20240201</creationdate><title>Profiling the Phytochemicals of IOrostachys margaritifolia/I: Biological Activities, LC-ESI/MS, and HPLC Analyses</title><author>Yoon, Nari ; Yu, Hyeonjun ; Tran, Gia Han ; Ko, Chung Ho ; Kim, Hoon ; Yoon, Mi Jeong ; Lee, Sanghyun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g677-ce758b9a22ff82cbc04140c0b11260743b3a8e831051b36759d35970fe4d8a013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Anti-inflammatory drugs</topic><topic>Chemical properties</topic><topic>Crassulaceae</topic><topic>Health aspects</topic><topic>High performance liquid chromatography</topic><topic>Identification and classification</topic><topic>Mass spectrometry</topic><topic>Pharmaceutical research</topic><topic>Phytochemicals</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yoon, Nari</creatorcontrib><creatorcontrib>Yu, Hyeonjun</creatorcontrib><creatorcontrib>Tran, Gia Han</creatorcontrib><creatorcontrib>Ko, Chung Ho</creatorcontrib><creatorcontrib>Kim, Hoon</creatorcontrib><creatorcontrib>Yoon, Mi Jeong</creatorcontrib><creatorcontrib>Lee, Sanghyun</creatorcontrib><jtitle>Pharmaceuticals (Basel, Switzerland)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yoon, Nari</au><au>Yu, Hyeonjun</au><au>Tran, Gia Han</au><au>Ko, Chung Ho</au><au>Kim, Hoon</au><au>Yoon, Mi Jeong</au><au>Lee, Sanghyun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Profiling the Phytochemicals of IOrostachys margaritifolia/I: Biological Activities, LC-ESI/MS, and HPLC Analyses</atitle><jtitle>Pharmaceuticals (Basel, Switzerland)</jtitle><date>2024-02-01</date><risdate>2024</risdate><volume>17</volume><issue>3</issue><issn>1424-8247</issn><eissn>1424-8247</eissn><abstract>Orostachys margaritifolia Y. N. Lee (OMY) is an endemic Korean plant in the family Crassulaceae that is known to contain a variety of bioactive compounds. To assess the physiological activities of an OMY ethanol extract, ABTS[sup.+] and DPPH radical scavenging assays and a nitric oxide (NO) inhibition assay were conducted. The phytochemical makeup of the extract was profiled via liquid chromatography-mass spectrometry (LC-ESI/MS) and high-performance liquid chromatography with a photodiode array detector (HPLC/PDA). The OMY extract was found to have weaker ABTS[sup.+] and DPPH radical scavenging activities than the control group (green tea). In the NO inhibition assay, the OMY extract induced a significant increase in macrophage cell viability but showed a lower NO inhibitory activity than l-NAME, producing an IC[sub.50] value of 202.6 μg/mL. The LC-ESI/MS and HPLC/PDA analyses identified isoquercitrin and astragalin in the OMY extract, quantifying their contents at 3.74 mg/g and 3.19 mg/g, respectively. The study revealed possibilities for the utilization of OMY as a future source of drugs for alleviating inflammation and diseases related to reactive oxygen species.</abstract><pub>MDPI AG</pub><doi>10.3390/ph17030290</doi></addata></record> |
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subjects | Anti-inflammatory drugs Chemical properties Crassulaceae Health aspects High performance liquid chromatography Identification and classification Mass spectrometry Pharmaceutical research Phytochemicals |
title | Profiling the Phytochemicals of IOrostachys margaritifolia/I: Biological Activities, LC-ESI/MS, and HPLC Analyses |
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