Co-Culture of IP/I. Igingivalis/I and IF/I. Inucleatum/I Synergistically Elevates IL-6 Expression via TLR4 Signaling in Oral Keratinocytes

Periodontitis, characterized by persistent inflammation in the periodontium, is intricately connected to systemic diseases, including oral cancer. Bacteria, such as Porphyromonas gingivalis and Fusobacterium nucleatum, play a pivotal role in periodontitis development because they contribute to dysbi...

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Bibliographic Details
Published in:International journal of molecular sciences 2024-04, Vol.25 (7)
Main Authors: Yáñez, Lucas, Soto, Cristopher, Tapia, Héctor, Pacheco, Martín, Tapia, Javiera, Osses, Gabriela, Salinas, Daniela, Rojas-Celis, Victoria, Hoare, Anilei, Quest, Andrew F. G, Díaz-Elizondo, Jessica, Pérez-Donoso, José Manuel, Bravo, Denisse
Format: Article
Language:English
Subjects:
Bacteria
Development and progression
Inflammation
Virulence (Microbiology)
Online Access:Get full text
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Summary:Periodontitis, characterized by persistent inflammation in the periodontium, is intricately connected to systemic diseases, including oral cancer. Bacteria, such as Porphyromonas gingivalis and Fusobacterium nucleatum, play a pivotal role in periodontitis development because they contribute to dysbiosis and tissue destruction. Thus, comprehending the interplay between these bacteria and their impacts on inflammation holds significant relevance in clinical understanding and treatment advancement. In the present work, we explored, for the first time, their impacts on the expressions of pro-inflammatory mediators after infecting oral keratinocytes (OKs) with a co-culture of pre-incubated P. gingivalis and F. nucleatum. Our results show that the co-culture increases IL-1β, IL-8, and TNF-α expressions, synergistically augments IL-6, and translocates NF-kB to the cell nucleus. These changes in pro-inflammatory mediators—associated with chronic inflammation and cancer—correlate with an increase in cell migration following infection with the co-cultured bacteria or P. gingivalis alone. This effect depends on TLR4 because TLR4 knockdown notably impacts IL-6 expression and cell migration. Our study unveils, for the first time, crucial insights into the outcomes of their co-culture on virulence, unraveling the role of bacterial interactions in polymicrobial diseases and potential links to oral cancer.
ISSN:1422-0067
DOI:10.3390/ijms25073611