High-Resolution Cryo-Electron Microscopy Structure Determination of IHaemophilus influenzae/I Tellurite-Resistance Protein A via 200 kV Transmission Electron Microscopy

• Published in: International journal of molecular sciences 2024-04, Vol.25 (8)
• Main Authors: Tran, Nhi L, Senko, Skerdi, Lucier, Kyle W, Farwell, Ashlyn C, Silva, Sabrina M, Dip, Phat V, Poweleit, Nicole, Scapin, Giovanna, Catalano, Claudio
• Format: Article
• Language: English
• Subjects: Detergents, Synthetic; Electron microscopy; Hemophilus infections; Membrane proteins; Sugars
• ISSN: 1422-0067
• DOI: 10.3390/ijms25084528

Membrane proteins constitute about 20% of the human proteome and play crucial roles in cellular functions. However, a complete understanding of their structure and function is limited by their hydrophobic nature, which poses significant challenges in purification and stabilization. Detergents, essential in the isolation process, risk destabilizing or altering the proteins’ native conformations, thus affecting stability and functionality. This study leverages single-particle cryo-electron microscopy to elucidate the structural nuances of membrane proteins, focusing on the SLAC1 bacterial homolog from Haemophilus influenzae (HiTehA) purified with diverse detergents, including n-dodecyl β-D-maltopyranoside (DDM), glycodiosgenin (GDN), β-D-octyl-glucoside (OG), and lauryl maltose neopentyl glycol (LMNG). This research not only contributes to the understanding of membrane protein structures but also addresses detergent effects on protein purification. By showcasing that the overall structural integrity of the channel is preserved, our study underscores the intricate interplay between proteins and detergents, offering insightful implications for drug design and membrane biology.