UHPLC-HRMS/MS Chemical Fingerprinting of the Bioactive Partition from Cultivated IPiper aduncum/I L

Piper aduncum L. is widely distributed in tropical regions and the ethnobotanical uses of this species encompass medicinal applications for the treatment of respiratory, antimicrobial, and gynecological diseases. Chemical studies reveal a diverse array of secondary metabolites, including terpenes, f...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2024-04, Vol.29 (8)
Main Authors: de Luna, Adélia Viviane, Fagundes, Thayssa da Silva Ferreira, Ramos, Ygor Jessé, de Araújo, Marlon Heggdorne, Muzitano, Michelle Frazão, Calixto, Sanderson Dias, Simão, Thatiana Lopes Biá Ventura, de Queiroz, George Azevedo, Guimarães, Elsie Franklin, Marques, André Mesquita, Moreira, Davyson de Lima
Format: Article
Language:English
Subjects:
Benzoic acid
Care and treatment
Fatty acids
Isoflavones
Medical research
Medicine, Botanic
Medicine, Experimental
Medicine, Herbal
Organic compounds
Plant metabolites
Tuberculosis
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Summary:Piper aduncum L. is widely distributed in tropical regions and the ethnobotanical uses of this species encompass medicinal applications for the treatment of respiratory, antimicrobial, and gynecological diseases. Chemical studies reveal a diverse array of secondary metabolites, including terpenes, flavonoids, and prenylated compounds. Extracts from P. aduncum have shown antibacterial, antifungal, and larvicidal activities. Our study explores the activity of extracts and partitions against Mycobacterium tuberculosis H37Rv, as well as the chemical diversity of the bioactive partition. This marks the first investigation of the bioactive partition of P. aduncum from agroecological cultivation. The ethyl acetate partition from the ethanolic leaf extract (PAEPL) was found to be the most active. PAEPL was subjected to column chromatography using Sephadex LH-20 and the obtained fractions were analyzed using UHPLC-HRMS/MS. The MS/MS data from the fractions were submitted to the online GNPS platform for the generation of the molecular network, which displayed 1714 nodes and 167 clusters. Compounds were identified via manual inspection and different libraries, allowing the annotation of 83 compounds, including flavonoids, benzoic acid derivatives, glycosides, free fatty acids, and glycerol-esterified fatty acids. This study provides the first chemical fingerprint of an antimycobacterial sample from P. aduncum cultivated in an agroecological system.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules29081690