Ultrasensitive Ti[sub.3]C[sub.2]Tx@Pt-Based Immunochromatography with Catalytic Amplification and a Dual Signal for the Detection of Chloramphenicol in Animal-Derived Foods

• Published in: Foods 2024-05, Vol.13 (9)
• Main Authors: Lin, Mengfang, Gao, Zhimin, Qian, Zhenjie, Deng, Youwen, Chen, Yanhong, Wang, Yu, Li, Xiangmei
• Format: Article
• Language: English
• Subjects: Chloramphenicol; Food; Livestock; Poultry industry; Safety and security measures
• ISSN: 2304-8158; 2304-8158
• DOI: 10.3390/foods13091416

Herein, a catalytic amplification enhanced dual-signal immunochromatographic assay (ICA) based on Pt nanoparticles (Pt NPs) modified with Ti[sub.3]C[sub.2]Tx MXene (Ti[sub.3]C[sub.2]Tx@Pt) was first developed for chloramphenicol (CAP) in animal-derived foods. Due to the large specific surface area and abundant active sites of Ti[sub.3]C[sub.2]Tx@Pt, they can be loaded with hundreds of Pt NPs to enhance their catalytic activity, resulting in a significant increase in the detection sensitivity; the sensitivity was up to 50-fold more sensitive than the reported ICA for CAP. The LODs of the developed method for milk/chicken/fish were 0.01 μg/kg, the LOQs were 0.03 μg/kg and the recovery rates were 80.5–117.0%, 87.2–118.1% and 92.7–117.9%, with corresponding variations ranging from 3.1 to 9.6%, 6.0 to 12.7% and 6.0 to 13.6%, respectively. The linear range was 0.0125–1.0 μg/kg. The results of the LC-MS/MS confirmation test on 30 real samples had a good correlation with that of our established method (R[sup.2] > 0.98), indicating the practical reliability of the established method. The above results indicated that an ICA based on the Ti[sub.3]C[sub.2]Tx@Pt nanozyme has excellent potential as a food safety detection tool.