Metallo-Glycodendrimeric Materials against Enterotoxigenic IEscherichia coli/I

Conjugation of carbohydrates to nanomaterials has been extensively studied and recognized as an alternative in the biomedical field. Dendrimers synthesized with mannose at the end group and with entrapped zero-valent copper/silver could be a potential candidate against bacterial proliferation. This...

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Bibliographic Details
Published in:Microorganisms (Basel) 2024-05, Vol.12 (5)
Main Authors: El Riz, Aly, Tchoumi Neree, Armelle, Mousavifar, Leila, Roy, René, Chorfi, Younes, Mateescu, Mircea Alexandru
Format: Article
Language:English
Subjects:
Antibacterial agents
Carbohydrates
Chemical properties
Control
Dendrimers
Escherichia coli infections
Health aspects
Materials research
Nanotechnology
Transition metals
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Summary:Conjugation of carbohydrates to nanomaterials has been extensively studied and recognized as an alternative in the biomedical field. Dendrimers synthesized with mannose at the end group and with entrapped zero-valent copper/silver could be a potential candidate against bacterial proliferation. This study is aimed at investigating the bactericidal activity of metal-glycodendrimers. The Cu(I)-catalyzed azide–alkyne cycloaddition (CuAAC) reaction was used to synthesize a new mannosylated dendrimer containing 12 mannopyranoside residues in the periphery. The enterotoxigenic Escherichia coli fimbriae 4 (ETEC:F4) viability, measured at 600 nm, showed the half-inhibitory concentration (IC[sub.50] ) of metal-free glycodendrimers (D), copper-loaded glycodendrimers (D:Cu) and silver-loaded glycodendrimers (D:Ag) closed to 4.5 × 10[sup.1] , 3.5 × 10[sup.1] and to 1.0 × 10[sup.−2] µg/mL, respectively, and minimum inhibitory concentration (MIC) of D, D:Cu and D:Ag of 2.0, 1.5 and 1.0 × 10[sup.−4] µg/mL, respectively. The release of bacteria contents onto broth and the inhibition of ETEC:F4 biofilm formation increased with the number of metallo-glycodendrimer materials, with a special interest in silver-containing nanomaterial, which had the highest activity, suggesting that glycodendrimer-based materials interfered with bacteria-bacteria or bacteria–polystyrene interactions, with bacteria metabolism and can disrupt bacteria cell walls. Our findings identify metal–mannose-dendrimers as potent bactericidal agents and emphasize the effect of entrapped zero-valent metal against ETEC:F4.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms12050966