IFodinisporobacter ferrooxydans/I gen. nov., sp. nov.—A Spore-Forming Ferrous-Oxidizing Bacterium Isolated from a Polymetallic Mine

A novel acidophilic, aerobic bacterium strain, MYW30-H2[sup.T] , was isolated from a heap of polymetallic mine. Cells of strain MYW30-H2[sup.T] were Gram-stain-positive, endospore-forming, motile, and rod-shaped. Strain MYW30-H2[sup.T] grew at a temperature range of 30–45 °C (optimum 40 °C) and a pH...

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Bibliographic Details
Published in:Microorganisms (Basel) 2024-05, Vol.12 (5)
Main Authors: Jiang, Zhen, Li, Xiutong, Liang, Zonglin, Tan, Zebao, Zhou, Nan, Liu, Ying, Liu, Zhenghua, Yin, Huaqun, Luo, Kun, Ingsriswang, Supawadee, Liu, Shuangjiang, Jiang, Chengying
Format: Article
Language:English
Subjects:
Bacteria, Aerobic
DNA sequencing
Genetic aspects
Identification and classification
Methods
Natural history
Nucleotide sequencing
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Summary:A novel acidophilic, aerobic bacterium strain, MYW30-H2[sup.T] , was isolated from a heap of polymetallic mine. Cells of strain MYW30-H2[sup.T] were Gram-stain-positive, endospore-forming, motile, and rod-shaped. Strain MYW30-H2[sup.T] grew at a temperature range of 30–45 °C (optimum 40 °C) and a pH range of 3.5–6.0 (optimum 4.0) in the presence of 0–0.5% (w /v ) NaCl. Strain MYW30-H2[sup.T] could grow heterotrophically on yeast extract and glucose, and grow mixotrophically using ferrous iron as an electron donor with yeast extract. Menaquinone-7 (MK-7) was the sole respiratory quinone of the strain. Iso-C[sub.15:0] and anteiso-C[sub.15:0] were the major cellular fatty acids. The 16S rRNA gene sequence analysis showed that MYW30-H2[sup.T] was phylogenetically affiliated with the family Alicyclobacillaceae , and the sequence similarity with other Alicyclobacillaceae genera species was below 91.51%. The average amino acid identity value of the strain with its phylogenetically related species was 52.3–62.1%, which fell into the genus boundary range. The DNA G+C content of the strain was 44.2%. Based on physiological and phylogenetic analyses, strain MYW30-H2[sup.T] represents a novel species of a new genus of the family Alicyclobacillaceae , for which the name Fodinisporobacter ferrooxydans gen. nov., sp. nov. is proposed. The type strain is MYW30-H2[sup.T] (=CGMCC 1.17422[sup.T] = KCTC 43278[sup.T] ).
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms12050853