Influenza A virus surveillance in domestic pigs in Kazakhstan 2018-2021/Vigilancia do virus influenza A em suinos domesticos no Cazaquistao 2018-2021

• Published in: Ciência rural 2024-10, Vol.54 (10), p.59
• Main Authors: Lukmanova, Galina, Klivleyeva, Nailya, Glebova, Tatyana, Ongarbayeva, Nuray, Shamenova, Mira, Saktaganov, Nurbol, Baimukhametova, Assem, Baiseiit, Sagadat, Ismagulova, Dariya, Ismailov, Eldar, Baisseyev, Galikhan, Mustafin, Muafik
• Format: Article
• Language: English
• ISSN: 0103-8478
• DOI: 10.1590/0103-8478cr20230403

This study described the results of a surveillance program monitoring circulation of influenza A viruses among domestic pigs (Sus domesticus) in Kazakhstan during 2018-2021. PCR data derived from 2,513 samples (nasopharyngeal swabs) collected from swine on large pig complexes and peasant farms located in different regions of Kazakhstan revealed that about 5% of samples were positive for influenza A virus RNA. This result suggested low levels of influenza A virus circulation in Kazakhstan. Subtyping of a set of samples revealed that the main strains circulating in 2018-2019 were A/H1N1 and A/H3N2. Surveillance conducted in 2020-2021 identified only A/H1N1 viruses in swine. The PCR data were confirmed by isolation of six strains: five influenza A/H1N1 viruses and one A/H3N2 virus. Key words: swine influenza, influenza viruses, PCR screening, reassortants. Este estudo descreve os resultados de um programa de vigilancia que monitoriza a circulacao do virus influenza A entre suinos domesticos (Sus domesticus) no Cazaquistao durante 2018-2021. Os dados de PCR derivados de 2.513 amostras (zaragatoas nasofaringeas) recolhidas de suinos em grandes complexos de suinos e exploracoes camponesas localizadas em diferentes regioes do Cazaquistao revelaram que cerca de 5% das amostras foram positivas para RNA do virus influenza A. Este resultado sugere baixos niveis de circulacao do virus influenza Ano Cazaquistao. Asubtipagem de um conjunto de amostras revelou que as principais cepas circulantes em 2018-2019 foram A/H1N1 e A/H3N2. A vigilancia realizada em 2020-2021 identificou apenas virus A/H1N1 em suinos. Os dados de PCR foram confirmados pelo isolamento de seis cepas: cinco virus influenza A/H1N1 e um virus A/H3N2. Palavras-chave: influenza suina, virus influenza, triagem por PCR, rearranjos.