Characterization of GQA as a novel [beta]-lactamase inhibitor of CTX-M-15 and KPC-2 enzymes

[beta]-lactam resistance is a significant global public health issue. Outbreaks of bacteria resistant to extended-spectrum [beta]-lactams and carbapenems are serious health concerns that not only complicate medical care but also impact patient outcomes. The primary objective of this work was to expr...

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Bibliographic Details
Published in:Microbial cell factories 2024-08, Vol.23 (1)
Main Authors: Al-Madboly, Lamiaa A, El-Salam, Mohamed A. Abd, Bastos, Jairo K, Aboukhatwa, Shaimaa, El-Morsi, Rasha M
Format: Article
Language:English
Subjects:
Amino acids
Antibacterial agents
Bacterial pneumonia
Beta lactam antibiotics
Beta lactamases
Computer software industry
Drug resistance in microorganisms
Escherichia coli
Health aspects
Hydrolysis
Identification and classification
International economic relations
Pharmaceutical research
Pneumonia
Tannins
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Summary:[beta]-lactam resistance is a significant global public health issue. Outbreaks of bacteria resistant to extended-spectrum [beta]-lactams and carbapenems are serious health concerns that not only complicate medical care but also impact patient outcomes. The primary objective of this work was to express and purify two soluble recombinant representative serine [beta]-lactamases using Escherichia coli strain as an expression host and pET101/D as a cloning vector. Furthermore, a second objective was to evaluate the potential, innovative, and safe use of galloylquinic acid (GQA) from Copaifera lucens as a potential [beta]-lactamase inhibitor.
ISSN:1475-2859
1475-2859
DOI:10.1186/s12934-024-02421-1