Characterization of GQA as a novel [beta]-lactamase inhibitor of CTX-M-15 and KPC-2 enzymes

[beta]-lactam resistance is a significant global public health issue. Outbreaks of bacteria resistant to extended-spectrum [beta]-lactams and carbapenems are serious health concerns that not only complicate medical care but also impact patient outcomes. The primary objective of this work was to expr...

Full description

Saved in:
Bibliographic Details
Published in:Microbial cell factories 2024-08, Vol.23 (1)
Main Authors: Al-Madboly, Lamiaa A, El-Salam, Mohamed A. Abd, Bastos, Jairo K, Aboukhatwa, Shaimaa, El-Morsi, Rasha M
Format: Article
Language:English
Subjects:
Amino acids
Antibacterial agents
Bacterial pneumonia
Beta lactam antibiotics
Beta lactamases
Computer software industry
Drug resistance in microorganisms
Escherichia coli
Health aspects
Hydrolysis
Identification and classification
International economic relations
Pharmaceutical research
Pneumonia
Tannins
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page
container_issue 1
container_start_page
container_title Microbial cell factories
container_volume 23
creator Al-Madboly, Lamiaa A
El-Salam, Mohamed A. Abd
Bastos, Jairo K
Aboukhatwa, Shaimaa
El-Morsi, Rasha M
description [beta]-lactam resistance is a significant global public health issue. Outbreaks of bacteria resistant to extended-spectrum [beta]-lactams and carbapenems are serious health concerns that not only complicate medical care but also impact patient outcomes. The primary objective of this work was to express and purify two soluble recombinant representative serine [beta]-lactamases using Escherichia coli strain as an expression host and pET101/D as a cloning vector. Furthermore, a second objective was to evaluate the potential, innovative, and safe use of galloylquinic acid (GQA) from Copaifera lucens as a potential [beta]-lactamase inhibitor.
doi_str_mv 10.1186/s12934-024-02421-1
format article
fullrecord <record><control><sourceid>gale</sourceid><recordid>TN_cdi_gale_infotracmisc_A804328918</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A804328918</galeid><sourcerecordid>A804328918</sourcerecordid><originalsourceid>FETCH-LOGICAL-g1018-4d3bbc0a992f638c160e6040cca1f3742d87b3f978d4e33906cc8dc3e62c9d5c3</originalsourceid><addsrcrecordid>eNptz0tLAzEQAOAgCtbqH_AU8OQhNa_dTY5l0Vqs-KogFCnZPNrIbhaaWLS_3vVxaEGGYYbhm4EB4JTgASEiv4iESsYRpj9JCSJ7oEd4kSEqMrm_1R-CoxjfMCaFKFgPzMqlWimd7MpvVPJtgK2Do4chVBEqGNq1reGsskm9orpjqlHRQh-WvvKpXX3jcvqCbhHJoAoG3tyXiEIbNp-NjcfgwKk62pO_2gfPV5fT8hpN7kbjcjhBC4KJQNywqtJYSUldzoQmObY55lhrRRwrODWiqJiThTDcMiZxrrUwmtmcamkyzfrg7PfuQtV27oNrU_dS46OeDwXmjApJRKcG_6gujG28boN1vpvvLJzvLHQm2Y-0UO8xzsdPj9v2Cx6CcE4</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Characterization of GQA as a novel [beta]-lactamase inhibitor of CTX-M-15 and KPC-2 enzymes</title><source>Publicly Available Content Database</source><source>PubMed Central</source><creator>Al-Madboly, Lamiaa A ; El-Salam, Mohamed A. Abd ; Bastos, Jairo K ; Aboukhatwa, Shaimaa ; El-Morsi, Rasha M</creator><creatorcontrib>Al-Madboly, Lamiaa A ; El-Salam, Mohamed A. Abd ; Bastos, Jairo K ; Aboukhatwa, Shaimaa ; El-Morsi, Rasha M</creatorcontrib><description>[beta]-lactam resistance is a significant global public health issue. Outbreaks of bacteria resistant to extended-spectrum [beta]-lactams and carbapenems are serious health concerns that not only complicate medical care but also impact patient outcomes. The primary objective of this work was to express and purify two soluble recombinant representative serine [beta]-lactamases using Escherichia coli strain as an expression host and pET101/D as a cloning vector. Furthermore, a second objective was to evaluate the potential, innovative, and safe use of galloylquinic acid (GQA) from Copaifera lucens as a potential [beta]-lactamase inhibitor.</description><identifier>ISSN: 1475-2859</identifier><identifier>EISSN: 1475-2859</identifier><identifier>DOI: 10.1186/s12934-024-02421-1</identifier><language>eng</language><publisher>BioMed Central Ltd</publisher><subject>Amino acids ; Antibacterial agents ; Bacterial pneumonia ; Beta lactam antibiotics ; Beta lactamases ; Computer software industry ; Drug resistance in microorganisms ; Escherichia coli ; Health aspects ; Hydrolysis ; Identification and classification ; International economic relations ; Pharmaceutical research ; Pneumonia ; Tannins</subject><ispartof>Microbial cell factories, 2024-08, Vol.23 (1)</ispartof><rights>COPYRIGHT 2024 BioMed Central Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids></links><search><creatorcontrib>Al-Madboly, Lamiaa A</creatorcontrib><creatorcontrib>El-Salam, Mohamed A. Abd</creatorcontrib><creatorcontrib>Bastos, Jairo K</creatorcontrib><creatorcontrib>Aboukhatwa, Shaimaa</creatorcontrib><creatorcontrib>El-Morsi, Rasha M</creatorcontrib><title>Characterization of GQA as a novel [beta]-lactamase inhibitor of CTX-M-15 and KPC-2 enzymes</title><title>Microbial cell factories</title><description>[beta]-lactam resistance is a significant global public health issue. Outbreaks of bacteria resistant to extended-spectrum [beta]-lactams and carbapenems are serious health concerns that not only complicate medical care but also impact patient outcomes. The primary objective of this work was to express and purify two soluble recombinant representative serine [beta]-lactamases using Escherichia coli strain as an expression host and pET101/D as a cloning vector. Furthermore, a second objective was to evaluate the potential, innovative, and safe use of galloylquinic acid (GQA) from Copaifera lucens as a potential [beta]-lactamase inhibitor.</description><subject>Amino acids</subject><subject>Antibacterial agents</subject><subject>Bacterial pneumonia</subject><subject>Beta lactam antibiotics</subject><subject>Beta lactamases</subject><subject>Computer software industry</subject><subject>Drug resistance in microorganisms</subject><subject>Escherichia coli</subject><subject>Health aspects</subject><subject>Hydrolysis</subject><subject>Identification and classification</subject><subject>International economic relations</subject><subject>Pharmaceutical research</subject><subject>Pneumonia</subject><subject>Tannins</subject><issn>1475-2859</issn><issn>1475-2859</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNptz0tLAzEQAOAgCtbqH_AU8OQhNa_dTY5l0Vqs-KogFCnZPNrIbhaaWLS_3vVxaEGGYYbhm4EB4JTgASEiv4iESsYRpj9JCSJ7oEd4kSEqMrm_1R-CoxjfMCaFKFgPzMqlWimd7MpvVPJtgK2Do4chVBEqGNq1reGsskm9orpjqlHRQh-WvvKpXX3jcvqCbhHJoAoG3tyXiEIbNp-NjcfgwKk62pO_2gfPV5fT8hpN7kbjcjhBC4KJQNywqtJYSUldzoQmObY55lhrRRwrODWiqJiThTDcMiZxrrUwmtmcamkyzfrg7PfuQtV27oNrU_dS46OeDwXmjApJRKcG_6gujG28boN1vpvvLJzvLHQm2Y-0UO8xzsdPj9v2Cx6CcE4</recordid><startdate>20240808</startdate><enddate>20240808</enddate><creator>Al-Madboly, Lamiaa A</creator><creator>El-Salam, Mohamed A. Abd</creator><creator>Bastos, Jairo K</creator><creator>Aboukhatwa, Shaimaa</creator><creator>El-Morsi, Rasha M</creator><general>BioMed Central Ltd</general><scope>ISR</scope></search><sort><creationdate>20240808</creationdate><title>Characterization of GQA as a novel [beta]-lactamase inhibitor of CTX-M-15 and KPC-2 enzymes</title><author>Al-Madboly, Lamiaa A ; El-Salam, Mohamed A. Abd ; Bastos, Jairo K ; Aboukhatwa, Shaimaa ; El-Morsi, Rasha M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g1018-4d3bbc0a992f638c160e6040cca1f3742d87b3f978d4e33906cc8dc3e62c9d5c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Amino acids</topic><topic>Antibacterial agents</topic><topic>Bacterial pneumonia</topic><topic>Beta lactam antibiotics</topic><topic>Beta lactamases</topic><topic>Computer software industry</topic><topic>Drug resistance in microorganisms</topic><topic>Escherichia coli</topic><topic>Health aspects</topic><topic>Hydrolysis</topic><topic>Identification and classification</topic><topic>International economic relations</topic><topic>Pharmaceutical research</topic><topic>Pneumonia</topic><topic>Tannins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Al-Madboly, Lamiaa A</creatorcontrib><creatorcontrib>El-Salam, Mohamed A. Abd</creatorcontrib><creatorcontrib>Bastos, Jairo K</creatorcontrib><creatorcontrib>Aboukhatwa, Shaimaa</creatorcontrib><creatorcontrib>El-Morsi, Rasha M</creatorcontrib><collection>Gale In Context: Science</collection><jtitle>Microbial cell factories</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Al-Madboly, Lamiaa A</au><au>El-Salam, Mohamed A. Abd</au><au>Bastos, Jairo K</au><au>Aboukhatwa, Shaimaa</au><au>El-Morsi, Rasha M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of GQA as a novel [beta]-lactamase inhibitor of CTX-M-15 and KPC-2 enzymes</atitle><jtitle>Microbial cell factories</jtitle><date>2024-08-08</date><risdate>2024</risdate><volume>23</volume><issue>1</issue><issn>1475-2859</issn><eissn>1475-2859</eissn><abstract>[beta]-lactam resistance is a significant global public health issue. Outbreaks of bacteria resistant to extended-spectrum [beta]-lactams and carbapenems are serious health concerns that not only complicate medical care but also impact patient outcomes. The primary objective of this work was to express and purify two soluble recombinant representative serine [beta]-lactamases using Escherichia coli strain as an expression host and pET101/D as a cloning vector. Furthermore, a second objective was to evaluate the potential, innovative, and safe use of galloylquinic acid (GQA) from Copaifera lucens as a potential [beta]-lactamase inhibitor.</abstract><pub>BioMed Central Ltd</pub><doi>10.1186/s12934-024-02421-1</doi></addata></record>
fulltext fulltext
identifier ISSN: 1475-2859
ispartof Microbial cell factories, 2024-08, Vol.23 (1)
issn 1475-2859
1475-2859
language eng
recordid cdi_gale_infotracmisc_A804328918
source Publicly Available Content Database; PubMed Central
subjects Amino acids
Antibacterial agents
Bacterial pneumonia
Beta lactam antibiotics
Beta lactamases
Computer software industry
Drug resistance in microorganisms
Escherichia coli
Health aspects
Hydrolysis
Identification and classification
International economic relations
Pharmaceutical research
Pneumonia
Tannins
title Characterization of GQA as a novel [beta]-lactamase inhibitor of CTX-M-15 and KPC-2 enzymes
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T18%3A14%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characterization%20of%20GQA%20as%20a%20novel%20%5Bbeta%5D-lactamase%20inhibitor%20of%20CTX-M-15%20and%20KPC-2%20enzymes&rft.jtitle=Microbial%20cell%20factories&rft.au=Al-Madboly,%20Lamiaa%20A&rft.date=2024-08-08&rft.volume=23&rft.issue=1&rft.issn=1475-2859&rft.eissn=1475-2859&rft_id=info:doi/10.1186/s12934-024-02421-1&rft_dat=%3Cgale%3EA804328918%3C/gale%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-g1018-4d3bbc0a992f638c160e6040cca1f3742d87b3f978d4e33906cc8dc3e62c9d5c3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rft_galeid=A804328918&rfr_iscdi=true