Molecular Identification of Encephalitazoon intestinalis and the Prevalence of Renal Microsporidiosis in Renal Transplant Recipients in Turkiye/Renal Transplant Alicilarinda Renal Microsporidiosis Prevalansi ve Encephalitazoon intestinalis Molekuler Karakterizasyonu

Objective: In patients with end-stage kidney disease, kidney transplantation is the kidney replacement therapy option that provides the most successful survival. However, immunosuppression agents administered after kidney transplantation can increase the risk of opportunistic infections. Microsporid...

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Published in:Türkiye parazitolojii dergisi 2024-06, Vol.48 (2), p.111
Main Authors: Cetinkaya, Ulfet, Onal, Muge Gulcihan, Uysal, Cihan, Yel, Sibel, Basar, Merve, Dursun, Ismail, Sipahioglu, Murat Hayri
Format: Article
Language:English
Subjects:
Analysis
Care and treatment
Children
Chronic kidney failure
Epidemiology
Health aspects
Immunotherapy
Infection
Kidneys
Medical research
Medicine, Experimental
Organ transplant recipients
Phylogeny
RNA
Transplantation
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Summary:Objective: In patients with end-stage kidney disease, kidney transplantation is the kidney replacement therapy option that provides the most successful survival. However, immunosuppression agents administered after kidney transplantation can increase the risk of opportunistic infections. Microsporidia are obligate intracellular pathogens that can be fatal in immunosuppressed patients. The present study aimed to determine the prevalence of microsporidia in kidney transplantation recipients and the molecular characterization of the detected species. Methods: To evaluate the prevalence of renal microsporidiosis in kidney transplant recipients, the urine samples from a total of 325 patients were analyzed by real-time and nested polymerase chain reaction for Encephalitozoon spp. and Enterocytozoon bieneusi. Results: Only one (0.4%) sample from the adult patient was positive for the Encephalitozoon species, while no positivity was found in pediatric patients. It was determined as Encephalitozoon intestinalis by ITS rRNA gene region sequence analysis. A microsporidia species obtained from humans in Turkiye has been characterized for the first time and registered in GenBank. Conclusion: Our epidemiological results show that the prevalence of renal microsporidiosis in kidney transplant recipients is very low. In addition, as a result of the phylogenetic analysis of the detected isolate, it was observed that it was 100% identical to the isolates reported from dogs in Kayseri, Turkiye. This situation provided essential data regarding the zoonotic transmission dynamics of microsporidia. Keywords: Renal microsporidiosis, Encephalitozoon intestinalis, zoonotic transmission, kidney transplantation, Turkiye Amac: Bobrek nakli, son donem bobrek yetmezligi olan hastalarda en basarili sagkalim saglayan renal replasman tedavi secenegidir. Ancak bobrek nakli sonrasinda uygulanan immun baskilayici ajanlar firsatci enfeksiyon riskini artirmaktadir. Microsporidialar, immun sistemi baskilanmis hastalarda olumcul olabilen zorunlu hucre ici patojenlerdir. Bu calismada bobrek nakil hastalarinda microsporidia prevalansinin belirlenmesi ve tespit edilen turlerin molekuler karakterizasyonunun yapilmasi amaclandi. Yontemler: Bobrek nakli hastalarinda renal microsporidiosis prevalansini degerlendirmek icin toplam 325 hastadan alinan idrar ornekleri Encephalitozoon spp. ve Enterocytozoon bieneusi acisindan gercek zamanli ve nested polimeraz zincir reaksiyonu ile analiz edildi. Bulgular: Er
ISSN:1300-6320
DOI:10.4274/tpd.galenos.2024.05025