Evaluation of placental bed uterine in L-NAME-induced early-onset preeclampsia sican modelinde uterin plasental yatagin degerlendirilmesi

• Published in: Turkish journal of obstetrics and gynecology 2024-09, Vol.29 (3), p.180
• Main Authors: Fitriana, Fitriana, Soetrisno, Soetrisno, Sulistyowati, Sri, Indarto, Dono
• Format: Article
• Language: English
• Subjects: Care and treatment; Causes of; Health aspects; Mothers; Patient outcomes; Physiological aspects; Placenta; Preeclampsia
• ISSN: 2149-9322
• DOI: 10.4274/tjod.galenos.2024.99132

Objective: Preeclampsia (PE) is the leading cause of maternal death worldwide and is associated with long-term morbidity in both mothers and newborns. Animal modeling is considered a functional source for understanding PE pathogenesis, diagnostic standards, and therapeutic approaches. Materials and Methods: This study aimed to demonstrate and evaluate the use of N-nitro-L-arginine methyl ester (L-NAME) in a Wistar rat model under conditions similar to PE. A total of 12 rats were divided into 4 groups, each consisting of 3 members, including the pregnant control group and treatment groups administered low-dose (PE 25 mg/kg L-NAME/day), medium-dose (PE 50 mg/kg L-NAME/day), and high-dose L-NAME (PE 75 mg/kg L-NAME/day) L-NAME from gestational day 4 to 19. Measurements included blood pressure, creatinine, and proteinuria levels, placental histological changes, and placental tissue hypoxia-inducible factor 1-alpha, and plasma endothelial nitric oxide synthase levels. Results: The results showed that intervention with L-NAME at 75 mg/kg body weight/day (PE3) induced PE earlier than that with 50 mg/kg body weight/day L-NAME. Conclusion: The model conditions also support further research into PE pathogenesis. Keywords: eNOS, HIF1[alpha], L-NAME, MAP, preeclampsia, proteinuria, spiral arteries Ama : Preeklampsi (PE) d nya apinda anne l m n n nde gelen nedenidir ve hem annelerde hem de yenidoganlarda uzun s reli morbidite ile iliskilidir. Hayvan modelleri, PE patogenezini, tani standartlarini ve tedavi yaklasimlarini anlamak i in islevsel bir kaynak olarak kabul edilir. Gere ve Y ntemler: Bu alismada, N-nitro-L-arginin metil esterin (L-NAME) Wistar si an modelinde PE'ye benzer kosullar altinda kullaniminin g sterilmesi ve degerlendirilmesi ama landi. Gebeligin 4-14 g nlerinde olan toplam 12 si an, gebe kontrol grubu ve d s k doz L-NAME (PE 25 mg/kg L-NAME/g n), orta doz L-NAME (PE 50 mg/kg L-NAME/g n) ve y ksek doz L-NAME (PE 75 mg/kg L-NAME/g n) uygulanan tedavi gruplari dahil olmak zere her biri 3 yeden olusan 4 gruba ayrildi. l mler kan basincini, kreatinin ve protein ri d zeylerini, plasental histolojik verileri ve plasental doku hipoksisi ile ind klenen fakt r 1-alfa ve plazma endotelyal nitrik oksit sentaz seviyelerini i eriyordu. Bulgular: Sonu lar, 75 mg/kg v cut agirligi/g n (PE3) L-NAME m dahalesinin, 50 mg/kg v cut agirligi/g n L-NAME m dahalesinden daha erken PE'yi tetikledigini g sterdi. Sonu : Model kosullari ayni zamanda PE patogenezine y nelik daha