Biocontrol Activity of Bacillus altitudinis CH05 and Bacillus tropicus CH13 Isolated from Capsicum annuum L. Seeds against Fungal Strains

• Published in: Microorganisms (Basel) 2024-09, Vol.12 (10), p.1943
• Main Authors: Espinosa Bernal, Merle Ariadna, Mena Navarro, Mayra Paola, Arvizu Gómez, Jackeline Lizzeta, Saldaña, Carlos, Ramos López, Miguel Ángel, Amaro Reyes, Aldo, Escamilla García, Monserrat, Pacheco Aguilar, Juan Ramiro, Moreno, Victor Pérez, Rodríguez Morales, José Alberto, Álvarez Hidalgo, Erika, Nuñez Ramírez, Jorge, Hernández Flores, José Luis, Campos Guillén, Juan
• Format: Article
• Language: English
• Subjects: Acetoin; Bacillus; Bacillus (Bacteria); Bacillus altitudinis; Bacillus tropicus; Bacteria; biologic control; Biological control; Capsicum; Capsicum annuum; Chromosomes; Control; Dehydrogenases; Diseases and pests; Environmental aspects; Enzymes; Fermentation; Flowers & plants; Fungi; Fungi, Phytopathogenic; Fungicides; Fusarium oxysporum; Genomes; Genomic analysis; Laboratories; Metabolism; Metabolites; Microorganisms; Mycelia; Organic compounds; Peppers; Pesticides; Pests; phytopathogenic fungi; Plant diseases; Plant growth; Pyrazine; Relative abundance; Seeds; Strains (organisms); Sustainable agriculture; Sweet peppers; VOCs; volatile organic compound; Volatile organic compounds
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms12101943

In this study, seed-surface-associated bacteria from fresh fruits of spp. were analyzed to explore potential isolates for biocontrol of phytopathogenic fungal strains. A total of 76 bacterial isolates were obtained from three different species of chili pepper ( L., R. & P., and Jacq.), and two isolates were selected via mycelial growth inhibition assays based on their production of volatile organic compounds (VOCs) against six fungal strains. Genomic analysis identified these isolates as CH05, with a chromosome size of 3,687,823 bp and with 41.25% G+C, and CH13, with a chromosome size of 5,283,706 bp and with 35.24% G+C. Both bacterial strains showed high mycelial growth inhibition capacities against , sp., , and but lower inhibition capacities against and . VOC identification was carried out after 24 h of fermentation with 64 VOCs for CH05 and 53 VOCs for CH13. 2,5-Dimethyl pyrazine and acetoin had the highest relative abundance values in both bacterial strains. Our findings revealed that seed-surface-associated bacteria on spp. have the metabolic ability to produce VOCs for biocontrol of fungal strains and have the potential to be used in sustainable agriculture.