The 5-chlorouracil:7-deazaadenine base pair as an alternative to the dT:dA base pair

5-Chloro-2'-deoxyuridine as a possible component of a chemically modified genome has been discussed in terms of its influence on duplex stability and DNA polymerase incorporation properties. The search for its counterpart among different deoxyadenosine analogs (7-deaza-, 8-aza- and 8-aza-7-deaz...

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Bibliographic Details
Published in:Organic & biomolecular chemistry 2017, Vol.15 (1), p.168-176
Main Authors: Eremeeva, E, Abramov, M, Marlière, P, Herdewijn, P
Format: Article
Language:English
Subjects:
Life Sciences
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Summary:5-Chloro-2'-deoxyuridine as a possible component of a chemically modified genome has been discussed in terms of its influence on duplex stability and DNA polymerase incorporation properties. The search for its counterpart among different deoxyadenosine analogs (7-deaza-, 8-aza- and 8-aza-7-deaza-2'-deoxyadenosines) showed that the stable duplex formation as well as the synthesis of long constructs, more than 2 kb, were successful with the 5-chloro-2'-deoxyuridine and 7-deaza-2'-deoxyadenosine combination and with Taq DNA polymerase.
ISSN:1477-0520
1477-0539
DOI:10.1039/c6ob02274j