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CD10 and ICOS expression by multiparametric flow cytometry in angioimmunoblastic T-cell lymphoma

Angioimmunoblastic T-cell lymphoma is immunologically defined by the expression of CD10 and the follicular helper T cell (T FH ) markers such as CXCL13, programmed death-1 (PD-1) and inducible T-cell costimulator (ICOS). This T FH profile has been mainly reported by immunohistochemistry. Here, using...

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Bibliographic Details
Published in:Modern pathology 2011-07, Vol.24 (7), p.993-1003
Main Authors: Baseggio, Lucile, Traverse-Glehen, Alexandra, Berger, Françoise, Ffrench, Martine, Jallades, Laurent, Morel, Dominique, Goedert, Ghislaine, Magaud, Jean-Pierre, Salles, Gilles, Felman, Pascale
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Language:English
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Summary:Angioimmunoblastic T-cell lymphoma is immunologically defined by the expression of CD10 and the follicular helper T cell (T FH ) markers such as CXCL13, programmed death-1 (PD-1) and inducible T-cell costimulator (ICOS). This T FH profile has been mainly reported by immunohistochemistry. Here, using multiparametric flow cytometry, the relevance of ICOS and PD-1 to angioimmunoblastic T-cell lymphoma diagnosis was evaluated in lymph node ( n =15) as well as in peripheral blood ( n =13) among a series of 28 angioimmunoblastic T-cell lymphoma cases, in addition to the CD10 expression (available in 26 lymph node and 15 peripheral blood specimens). In this series, CD10 expression was present in 23/26 (88%) lymph node and in 12/15 (80%) peripheral blood cases and ICOS in 13/15 (87%) lymph node and in 6/13 (47%) peripheral blood cases, whereas neither significant CD10 nor ICOS T cells were identified in the control group (lymph nodes with reactive hyperplasia=10, peripheral blood of healthy donors=15). PD-1 expression was less informative as observed in both angioimmunoblastic T-cell lymphoma and control cases. The multiparametric approach allowed us to confirm the frequent blood dissemination in angioimmunoblastic T-cell lymphoma and to show that circulating neoplastic T cells correspond more often to a CD10-positive subset than to an ICOS-positive subset. Consequently, if ICOS constitutes an additional feature for the diagnosis of angioimmunoblastic T-cell lymphoma, it appears less sensitive than CD10 expression for the detection of circulating neoplastic T cells.
ISSN:0893-3952
1530-0285
DOI:10.1038/modpathol.2011.53