Johnson-Kendall-Roberts theory applied to living cells

Johnson-Kendall-Roberts (JKR) theory is an accurate model for strong adhesion energies of soft slightly deformable material. Little is known about the validity of this theory on complex systems such as living cells. We have addressed this problem using a depletion controlled cell adhesion and measur...

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Bibliographic Details
Published in:Physical review letters 2005-01, Vol.94 (2), p.028102-028102, Article 028102
Main Authors: Chu, Yeh-Shiu, Dufour, Sylvie, Thiery, Jean Paul, Perez, Eric, Pincet, Frederic
Format: Article
Language:English
Subjects:
Animals
Biological Physics
Cell Adhesion
Cell Line, Tumor
Cell Membrane
Computer Simulation
Cytoskeleton
Elasticity
Membrane Fluidity
Membrane Fusion
Mice
Micromanipulation - methods
Models, Biological
Physics
Sarcoma - pathology
Sarcoma - physiopathology
Stress, Mechanical
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Summary:Johnson-Kendall-Roberts (JKR) theory is an accurate model for strong adhesion energies of soft slightly deformable material. Little is known about the validity of this theory on complex systems such as living cells. We have addressed this problem using a depletion controlled cell adhesion and measured the force necessary to separate the cells with a micropipette technique. We show that the cytoskeleton can provide the cells with a 3D structure that is sufficiently elastic and has a sufficiently low deformability for JKR theory to be valid. When the cytoskeleton is disrupted, JKR theory is no longer applicable.
ISSN:0031-9007
1079-7114
DOI:10.1103/PhysRevLett.94.028102