Brain Expression of Tyrosine Hydroxylase and Its Regulation by Steroid Hormones in the European Eel Quantified by Real-Time PCR

: In the eel, dopamine inhibits pubertal development. To investigate the regulatory mechanisms involved, we developed a quantitative real‐time RT‐PCR assay for measurement of brain expression of tyrosine hydroxylase (TH), the rate‐limiting enzyme in the biosynthesis of dopamine. TH expression was hi...

Full description

Saved in:
Bibliographic Details
Published in:Annals of the New York Academy of Sciences 2005-04, Vol.1040 (1), p.518-520
Main Authors: WELTZIEN, F-A, PASQUALINI, C, LE BELLE, N, VIDAL, B, VERNIER, P, DUFOUR, S.
Format: Article
Language:English
Subjects:
Anguilla - metabolism
Animals
Biomedical materials
Brain
Brain - drug effects
Brain - enzymology
Dopamine
Elopomorpha
Enzymes
Female
Gene Expression Regulation, Enzymologic - drug effects
Gene Expression Regulation, Enzymologic - physiology
Gonadal Steroid Hormones - pharmacology
Gonadal Steroid Hormones - physiology
Hormones
Life Sciences
Neurons and Cognition
puberty
Real time
Reverse Transcriptase Polymerase Chain Reaction - methods
steroid feedback
Surgical implants
teleost
Tyrosine
Tyrosine 3-Monooxygenase - biosynthesis
Tyrosine 3-Monooxygenase - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:: In the eel, dopamine inhibits pubertal development. To investigate the regulatory mechanisms involved, we developed a quantitative real‐time RT‐PCR assay for measurement of brain expression of tyrosine hydroxylase (TH), the rate‐limiting enzyme in the biosynthesis of dopamine. TH expression was highest in the olfactory bulb, followed by the di‐/mesencephalic areas and the telencephalon/preoptic area. TH expression in the optic lobes and hindbrain was low or below the detection limit. In vivo treatment with testosterone, but not estradiol, resulted in increased TH expression in the forebrain, except the optic tectum, but not in the hindbrain. The results were confirmed by in situ hybridization.
ISSN:0077-8923
1749-6632
DOI:10.1196/annals.1327.108