An automated in situ hybridization screen in the medaka to identify unknown neural genes

Despite the fact that a large body of factors that play important roles in development are known, there are still large gaps in understanding the genetic pathways that govern these processes. To find previously unknown genes that are expressed during embryonic development, we optimized and performed...

Full description

Saved in:
Bibliographic Details
Published in:Developmental dynamics 2005-11, Vol.234 (3), p.698-708
Main Authors: Deyts, Carole, Candal, Eva, Joly, Jean‐Stephane, Bourrat, Franck
Format: Article
Language:English
Subjects:
Aging - physiology
Animals
Automation
Brain - metabolism
development
fish
Freshwater
Gene Expression Profiling
Gene Expression Regulation, Developmental
In Situ Hybridization
Life Sciences
medaka
Nerve Tissue Proteins - genetics
Nervous System - metabolism
Neurons and Cognition
Oryzias - genetics
Oryzias latipes
RNA, Messenger - genetics
unknown genes
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Despite the fact that a large body of factors that play important roles in development are known, there are still large gaps in understanding the genetic pathways that govern these processes. To find previously unknown genes that are expressed during embryonic development, we optimized and performed an automated whole‐mount in situ hybridization screen on medaka embryos at the end of somitogenesis. Partial cDNA sequences were compared against public databases and identified according to similarities found to other genes and gene products. Among 321 isolated genes showing specific expression in the central nervous system in at least one of five stages of development, 55.14% represented genes whose functions are already documented (in fish or other model organisms). Additionally, 16.51% were identified as conserved unknown genes or genes with unknown function. We provide new data on eight of these genes that presented a restricted expression pattern that allowed for formulating testable hypotheses on their developmental roles, and that were homologous to mammalian molecules of unknown function. Thus, gene expression screening in medaka is an efficient tool for isolating new regulators of embryonic development, and can complement genome‐sequencing projects that are producing a high number of genes without ascribed functions. Developmental Dynamics 234:698–708, 2005. © 2005 Wiley‐Liss, Inc.
ISSN:1058-8388
1097-0177
DOI:10.1002/dvdy.20465