Selective protein adsorption property and characterization of nano-crystalline zinc-containing hydroxyapatite

Nano-crystalline Zn-containing hydroxyapatite (ZnHAp) was prepared by the wet-chemical method and the selective adsorption of essential proteins was examined, taking bovine serum albumin (BSA) and pathogenic protein such as β 2-microglobulin (β 2-MG) as model proteins. Transmission electron microsco...

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Bibliographic Details
Published in:Acta biomaterialia 2006, Vol.2 (1), p.69-74
Main Authors: Fujii, Eiji, Ohkubo, Mizuki, Tsuru, Kanji, Hayakawa, Satoshi, Osaka, Akiyoshi, Kawabata, Koji, Bonhomme, Christian, Babonneau, Florence
Format: Article
Language:English
Subjects:
Adsorption
Albumin
Animals
beta 2-Microglobulin - chemistry
Biocompatible Materials - chemistry
Calcium phosphate
Cattle
Chemical Sciences
Crystallization
Humans
Hydroxyapatite
Hydroxyapatites - chemistry
In Vitro Techniques
Material chemistry
Materials Testing
Microscopy, Electron
Models, Biological
Nanostructures
Proteins - chemistry
Serum Albumin, Bovine - chemistry
Surface Properties
X-Ray Diffraction
Zinc
Zinc - chemistry
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Summary:Nano-crystalline Zn-containing hydroxyapatite (ZnHAp) was prepared by the wet-chemical method and the selective adsorption of essential proteins was examined, taking bovine serum albumin (BSA) and pathogenic protein such as β 2-microglobulin (β 2-MG) as model proteins. Transmission electron microscopy observation and X-ray diffraction analysis indicated that the increase of Zn content led to smaller crystallites and their specific surface area of ZnHAps increased with increasing Zn content, accordingly. Furthermore, the amounts of BSA adsorption on ZnHAp particles decreased with increasing Zn content in spite of the increase in the specific surface area. It is thus concluded that nano-crystalline ZnHAps had a highly selective adsorption property with regard to β 2-MG.
ISSN:1742-7061
1878-7568
DOI:10.1016/j.actbio.2005.09.002