NMR Metabolomics to Revisit the Tobacco Mosaic Virus Infection in Nicotiana tabacum Leaves

Tobacco mosaic virus (TMV) infection of tobacco is a well-known and extensively studied model system for which a number of genes and proteins involved in the systemic acquired resistance (SAR) have been characterized. Little is known about the metabolic changes connected with the infection and SAR....

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Bibliographic Details
Published in:Journal of natural products (Washington, D.C.) D.C.), 2006-05, Vol.69 (5), p.742-748
Main Authors: Choi, Y.H, Kim, H.K, Linthorst, H.J.M, Hollander, J.G, Lefeber, A.W.M, Erkelens, C, Nuzillard, J.M, Verpoorte, R
Format: Article
Language:English
Subjects:
alpha-Linolenic Acid - metabolism
Biological and medical sciences
Caffeic Acids - metabolism
Chlorogenic Acid - analogs & derivatives
Fundamental and applied biological sciences. Psychology
Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control
metabolomics
Models, Biological
Molecular Structure
Nicotiana - metabolism
Nicotiana - virology
Nicotiana tabacum
nuclear magnetic resonance spectroscopy
Nuclear Magnetic Resonance, Biomolecular
Phytopathology. Animal pests. Plant and forest protection
Plant Diseases - virology
Plant Leaves - chemistry
Plant Leaves - metabolism
plant viruses
Plant viruses and viroids
Quinic Acid - analogs & derivatives
Quinic Acid - metabolism
Terpenes - metabolism
Tobacco mosaic virus
Tobacco Mosaic Virus - pathogenicity
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Summary:Tobacco mosaic virus (TMV) infection of tobacco is a well-known and extensively studied model system for which a number of genes and proteins involved in the systemic acquired resistance (SAR) have been characterized. Little is known about the metabolic changes connected with the infection and SAR. Here we describe the use of NMR spectroscopy in combination with multivariate data analysis to study the metabolic changes. Particularly 2-D NMR methods, such as 2-D J-resolved spectra and their projected spectra, are shown to be powerful tools in the metabolomic studies. The macroscopic view of the metabolomes obtained by NMR spectroscopy of crude extracts enabled the identification of a series of totally different metabolites that seem connected with resistance, such as the clearly increased 5-caffeoylquinic acid, alpha-linolenic acid analogues, and sesqui- and diterpenoids in the infected plant parts.
ISSN:0163-3864
1520-6025
DOI:10.1021/np050535b