In silico identification and structural features of six new genes similar to MATER specifically expressed in the oocyte

In the present work, we have used the in silico subtraction methodology to identify six new mouse genes similar to NALP5/MATER, whose ESTs were represented almost exclusively in egg libraries. Five genes were selected for RT-PCR and/or in situ hybridization. These experiments confirmed their oocyte...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2004-11, Vol.324 (2), p.547-553
Main Authors: Dadé, Sébastien, Callebaut, Isabelle, Paillisson, Amélie, Bontoux, Martine, Dalbiès-Tran, Rozenn, Monget, Philippe
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antigens - genetics
Antigens - metabolism
Computer Science
DNA, Complementary - metabolism
Egg Proteins - genetics
Egg Proteins - metabolism
Expressed Sequence Tags
Expression
Gene Library
In silico
In Situ Hybridization
Life Sciences
MATER
Mice
Models, Genetic
Molecular Sequence Data
Mouse
Multigene Family
Oocyte
Oocytes - metabolism
Protein Structure, Tertiary
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
Sequence Analysis, DNA
Sequence Homology, Amino Acid
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Summary:In the present work, we have used the in silico subtraction methodology to identify six new mouse genes similar to NALP5/MATER, whose ESTs were represented almost exclusively in egg libraries. Five genes were selected for RT-PCR and/or in situ hybridization. These experiments confirmed their oocyte restricted expression. Five of these genes are localized on mouse chromosome 7, as is NALP5/MATER; among them, three are localized in a 300kb cluster.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2004.09.086