β‐Dystroglycan can be revealed in microsomes from mdx mouse muscle by detergent treatment

β‐Dystroglycan is the central member of a transmembrane protein complex of the skeletal muscle plasma membrane. Since it was not detected in dystrophin‐deficient skeletal muscles, a disruption of the complex was thought to be involved in the dystrophic process. We report here that β‐dystroglycan is...

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Bibliographic Details
Published in:FEBS letters 2004-08, Vol.572 (1-3), p.216-220
Main Authors: Cluchague, Nicolas, Moreau, Céline, Rocher, Chantal, Pottier, Sandrine, Leray, Geneviève, Cherel, Yan, Le Rumeur, Elisabeth
Format: Article
Language:English
Subjects:
Animals
Biochemistry
Biochemistry, Molecular Biology
Biomarkers - analysis
Biophysics
Cell Membrane - physiology
Cytoskeletal Proteins - analysis
Detergents
Dystroglycans
Dystrophin - analysis
Dystrophin-deficient muscle
Dystrophin–glycoprotein complex
Life Sciences
mdx mouse
Membrane Glycoproteins - analysis
Mice
Mice, Inbred mdx
Microsomes - physiology
Muscle, Skeletal - physiology
Muscular dystrophy
β-Dystroglycan
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Summary:β‐Dystroglycan is the central member of a transmembrane protein complex of the skeletal muscle plasma membrane. Since it was not detected in dystrophin‐deficient skeletal muscles, a disruption of the complex was thought to be involved in the dystrophic process. We report here that β‐dystroglycan is actually present at normal levels in mdx mouse muscle plasma membrane: treatment with cholate detergent is able to reveal its presence by SDS–PAGE and immunoblotting. This result shows that, in dystrophin‐deficient muscles, β‐dystroglycan is indeed targeted to the plasma membrane but remains inaccessible to classical solubilizing treatments and to antibodies used for immunolocalization.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2004.07.032