Mutation in phenol-type herbicide resistance maps within the psbA gene in Synechocystis 6714

A Synechocystis 6714 mutant resistant to the phenol-type herbicide ioxynil was isolated and characterized. Sensitivity to DCMU and atrazine was measured in whole cells and isolated thylakoids. The mutant presents the same sensitivity to atrazine as the wild type and a slightly increased sensitivity...

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Bibliographic Details
Published in:FEBS letters 1989-03, Vol.246 (1), p.207-210
Main Authors: Ajlani, G., Meyer, I., Vernotte, C., Astier, C.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacteriology
Base Sequence
Biochemistry, Molecular Biology
Chlorophyll
Chlorophyll - genetics
Cloning, Molecular
Codon
Cyanobacteria
Cyanobacteria - drug effects
Cyanobacteria - genetics
DCMU, 3-(3,4-dichlorophenyl)-1,1-dimethyl-urea
Drug Resistance, Microbial
Genes
Herbicide
Herbicides
Herbicides - pharmacology
Iodobenzenes
Iodobenzenes - pharmacology
Ioxynil
Life Sciences
Light-Harvesting Protein Complexes
Microbiology and Parasitology
Molecular biology
Molecular Sequence Data
Mutant
Mutation
Nitriles
Nitriles - pharmacology
Photosynthetic Reaction Center Complex Proteins
Photosystem II
Plant Proteins
Plant Proteins - genetics
PS II, photosystem II
Synechocystis
Vegetal Biology
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Summary:A Synechocystis 6714 mutant resistant to the phenol-type herbicide ioxynil was isolated and characterized. Sensitivity to DCMU and atrazine was measured in whole cells and isolated thylakoids. The mutant presents the same sensitivity to atrazine as the wild type and a slightly increased sensitivity to DCMU. A point mutation has been found at codon 266 in the psbAI coding locus (AAC to ACC) resulting in an amino acid change from asparagine to threonine in the D 1 protein.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(89)80284-4