A chimeric human T cell leukemia virus type I bearing a deltaR Moloney-murine leukemia virus envelope infects mice persistently and induces humoral and cellular immune responses

Human T cell lymphotropic virus (HTLV) type I is the agent of adult T cell leukemia and HTLV-I-associated myelopathy. Because its pathogenesis is not well understood, a mouse model of HTLV-I infection would be valuable. We report the infection of adult BALB/c, C3H/He, 129Sv, and 129Sv IFNAR(-/-) mic...

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Bibliographic Details
Published in:The Journal of infectious diseases 2005-01, Vol.191 (2), p.255-263
Main Authors: Delebecque, Frédéric, Combredet, Chantal, Gabet, Anne-Sophie, Wattel, Eric, Brahic, Michel, Tangy, Frédéric
Format: Article
Language:English
Subjects:
Animals
Antibody Formation - immunology
Cell Line
Chimera - immunology
Disease Models, Animal
Human T-lymphotropic virus 1 - genetics
Human T-lymphotropic virus 1 - immunology
Humans
Immunity, Cellular - immunology
Life Sciences
Mice
Microbiology and Parasitology
Moloney murine leukemia virus - genetics
Moloney murine leukemia virus - immunology
Proviruses
Viral Envelope Proteins - genetics
Viral Envelope Proteins - immunology
Virology
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Summary:Human T cell lymphotropic virus (HTLV) type I is the agent of adult T cell leukemia and HTLV-I-associated myelopathy. Because its pathogenesis is not well understood, a mouse model of HTLV-I infection would be valuable. We report the infection of adult BALB/c, C3H/He, 129Sv, and 129Sv IFNAR(-/-) mice with an infectious chimeric HTLV-I provirus bearing the Moloney-murine leukemia virus (Mo-MuLV) envelope glycoprotein truncated for the C-terminal R peptide. Mice were persistently infected (500-800 proviral DNA copies/10(5) splenocytes) for at least 20 weeks after inoculation. The chimeric virus disseminated to several organs, such as spleen, thymus, lung, brain, and spinal cord. The amplification of proviral integration sites showed an oligoclonal integration resembling that reported in HTLV-I-infected humans. Infected mice developed lasting humoral and cellular immune responses. This DeltaR HTLV-I/Mo-MuLV chimeric virus, with the Mo-MuLV Env tropism and HTLV-I replication characteristics, could provide a mouse model of HTLV-I infection.
ISSN:0022-1899
1537-6613
DOI:10.1086/426825