Oligosaccharide synthesis in Fibrobacter succinogenes S85 and its modulation by the substrate

In this article we compared the metabolism of phosphorylated and unphosphorylated oligosaccharides (cellodextrins and maltodextrins) in Fibrobacter succinogenes S85 resting cells incubated with the following substrates: glucose; cellobiose; a mixture of glucose and cellobiose; and cellulose. Intrace...

Full description

Saved in:
Bibliographic Details
Published in:The FEBS journal 2005-05, Vol.272 (10), p.2416-2427
Main Authors: Nouaille, Régis, Matulova, Maria, Delort, Anne‐Marie, Forano, Evelyne
Format: Article
Language:English
Subjects:
Bacteria
Cellobiose - metabolism
cellodextrin
Cells, Cultured
Cellulose - analogs & derivatives
Cellulose - biosynthesis
Cellulose - chemistry
Cellulose - metabolism
Chemical synthesis
Comparative analysis
Culture Media - chemistry
Dextrins - biosynthesis
Dextrins - chemistry
Fibrobacter - cytology
Fibrobacter - metabolism
Fibrobacter succinogenes
Fibrobacter succinogenes S85
Glucose - metabolism
maltodextrin
Maltose - metabolism
Metabolism
NMR
Nuclear Magnetic Resonance, Biomolecular
Phosphorylation
Polysaccharides - biosynthesis
Polysaccharides - chemistry
rumen
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this article we compared the metabolism of phosphorylated and unphosphorylated oligosaccharides (cellodextrins and maltodextrins) in Fibrobacter succinogenes S85 resting cells incubated with the following substrates: glucose; cellobiose; a mixture of glucose and cellobiose; and cellulose. Intracellular and extracellular media were analysed by 1H‐NMR and by TLC. The first important finding is that no cellodextrins were found to accumulate in the extracellular media of cells, regardless of the substrate; this contrasts to what is generally reported in the literature. The second finding of this work is that maltodextrins of degree of polymerization > 2 are synthesized regardless of the substrate, and can be used by the bacteria. Maltotriose plays a key role in this metabolism of maltodextrin. Maltodextrin‐1‐phosphate was detected in all the incubations, and a new metabolite, corresponding to a phosphorylated glucose derivative, was produced in the extracellular medium when cells were incubated with cellulose. The accumulation of these phosphorylated sugars increased with the degree of polymerization of the substrate.
ISSN:1742-464X
1742-4658
DOI:10.1111/j.1742-4658.2005.04662.x