Effects of brefeldin A and nordihydroguaiaretic acid on endomembrane dynamics and lipid synthesis in plant cells

Effects of brefeldin A (BFA) and nordihydroguaiaretic acid (NDGA) on endomembrane structures and lipid synthesis were compared in maize root cells and tobacco Bright Yellow-2 cells. Immunofluorescence and electron microscopy studies showed that NDGA altered the structure and distribution of the endo...

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Bibliographic Details
Published in:FEBS letters 2002-05, Vol.518 (1), p.88-92
Main Authors: Mérigout, Patricia, Képès, François, Perret, Anne-Marie, Satiat-Jeunemaitre, Béatrice, Moreau, Patrick
Format: Article
Language:English
Subjects:
Brefeldin A
Brefeldin A - pharmacology
Cell Division
Cell Division - drug effects
Cells, Cultured
Dose-Response Relationship, Drug
Endoplasmic Reticulum
Endoplasmic Reticulum - drug effects
Endoplasmic Reticulum - ultrastructure
Golgi Apparatus
Golgi Apparatus - drug effects
Golgi Apparatus - physiology
Golgi Apparatus - ultrastructure
Golgi dynamics
Intracellular Membranes
Intracellular Membranes - drug effects
Intracellular Membranes - ultrastructure
Life Sciences
Lipids
Lipids - biosynthesis
Masoprocol - pharmacology
Nicotiana - drug effects
Nicotiana - metabolism
Nicotiana - ultrastructure
Nordihydroguaiaretic Acid
Phytosterols
Phytosterols - biosynthesis
Plants
Plants - drug effects
Sterol
Tobacco
Vegetal Biology
Zea mays
Zea mays - drug effects
Zea mays - metabolism
Zea mays - ultrastructure
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Summary:Effects of brefeldin A (BFA) and nordihydroguaiaretic acid (NDGA) on endomembrane structures and lipid synthesis were compared in maize root cells and tobacco Bright Yellow-2 cells. Immunofluorescence and electron microscopy studies showed that NDGA altered the structure and distribution of the endoplasmic reticulum (ER) within 1 h but not of the Golgi apparatus whereas, as shown previously, BFA altered that organization of the Golgi apparatus and, only subsequently, of the ER. Biochemical studies revealed that both drugs and especially BFA led to a strong inhibition of the phytosterol biosynthetic pathway: BFA led to accumulation of sterol precursors. The importance of phytosterols in membrane architecture and membrane trafficking is discussed.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(02)02651-0