The hepatitis C virus minigenome: A new cellular model for studying viral replication

The cellular models used usually to study hepatitis C virus replication involve coupling between translation and replication. Because this linkage makes detailed analyses difficult a new cellular model was developed where replication is rendered independent of translation. The RNA replication was st...

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Bibliographic Details
Published in:Journal of virological methods 2007-06, Vol.142 (1), p.59-66
Main Authors: Dumas, E., Masante, C., Astier-Gin, T., Lapaillerie, D., Ventura, M.
Format: Article
Language:English
Subjects:
3' Untranslated Regions - genetics
5' Untranslated Regions - genetics
Biological and medical sciences
Cell Line, Tumor
Fundamental and applied biological sciences. Psychology
Genes, Reporter
Genome, Viral
HCV
Hepacivirus - genetics
Hepacivirus - physiology
Hepatitis C virus
Humans
Life Sciences
Microbiology
Microbiology and Parasitology
Minigenome
Replication
Replicon
RNA, Viral - metabolism
Techniques used in virology
Transfection
Viral Nonstructural Proteins - genetics
Viral Nonstructural Proteins - metabolism
Virology
Virology - methods
Virus
Virus Replication
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Summary:The cellular models used usually to study hepatitis C virus replication involve coupling between translation and replication. Because this linkage makes detailed analyses difficult a new cellular model was developed where replication is rendered independent of translation. The RNA replication was studied using RNA minigenomes where the reporter gene was flanked by the two untranslated regions of HCV. It was shown that these RNA minigenomes could be stably replicated into Huh7 cells expressing the HCV replication complex. This was obtained either by constitutively expressing the non-structural proteins into Huh7 hepatoma cells or by using Huh7 cells harboring replicons.
ISSN:0166-0934
1879-0984
DOI:10.1016/j.jviromet.2007.01.005