Structure of the Exon Junction Core Complex with a Trapped DEAD-Box ATPase Bound to RNA

• Published in: Science (American Association for the Advancement of Science) 2006-09, Vol.313 (5795), p.1968-1972
• Main Authors: Andersen, Christian B.F, Ballut, Lionel, Johansen, Jesper S, Chamieh, Hala, Nielsen, Klaus H, Oliveira, Cristiano L.P, Pedersen, Jan Skov, Séraphin, Bertrand, Le Hir, Hervé, Andersen, Gregers Rom
• Format: Article
• Language: English
• Subjects: Adenosine triphosphatase; Adenosine triphosphatases; Adenosine Triphosphate; Adenosine Triphosphate - analogs & derivatives; Adenosine Triphosphate - metabolism; Adenylyl Imidodiphosphate; Adenylyl Imidodiphosphate - metabolism; Amino Acid Motifs; Amino Acid Sequence; Animals; Binding sites; Biochemistry, Molecular Biology; Biological and medical sciences; Crystal structure; Crystalline structure; Crystallography, X-Ray; DEAD-box RNA Helicases; Dimerization; Double stranded RNA; Drosophila Proteins; Drosophila Proteins - chemistry; Drosophila Proteins - metabolism; Eukaryotes; Eukaryotic Initiation Factor-4A; Eukaryotic Initiation Factor-4A - chemistry; Eukaryotic Initiation Factor-4A - metabolism; Exons; Fundamental and applied biological sciences. Psychology; Humans; Hydrogen Bonding; Hydrolysis; Life Sciences; Messenger RNA; Models, Molecular; Molecular biology; Molecular biophysics; Molecular Sequence Data; Molecules; Mutation; Neoplasm Proteins; Neoplasm Proteins - chemistry; Neoplasm Proteins - metabolism; Nuclear Proteins; Nuclear Proteins - chemistry; Nuclear Proteins - metabolism; Nucleic Acid Conformation; Nucleotides; Poly U; Poly U - chemistry; Poly U - metabolism; Protein Conformation; Protein Structure, Secondary; Protein Structure, Tertiary; Proteins; Ribonucleic acid; RNA; RNA Helicases; RNA Helicases - chemistry; RNA Helicases - metabolism; RNA, Messenger; RNA, Messenger - chemistry; RNA, Messenger - metabolism; RNA-Binding Proteins; RNA-Binding Proteins - chemistry; RNA-Binding Proteins - metabolism; Structure in molecular biology
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.1131981

In higher eukaryotes, a multiprotein exon junction complex is deposited on spliced messenger RNAs. The complex is organized around a stable core, which serves as a binding platform for numerous factors that influence messenger RNA function. Here, we present the crystal structure of a tetrameric exon junction core complex containing the DEAD-box adenosine triphosphatase (ATPase) eukaryotic initiation factor 4AIII (eIF4AIII) bound to an ATP analog, MAGOH, Y14, a fragment of MLN51, and a polyuracil mRNA mimic. eIF4AIII interacts with the phosphate-ribose backbone of six consecutive nucleotides and prevents part of the bound RNA from being double stranded. The MAGOH and Y14 subunits lock eIF4AIII in a prehydrolysis state, and activation of the ATPase probably requires only modest conformational changes in eIF4AIII motif I.