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Discrete localizations of hedgehog signalling components in the developing and adult rat nervous system

Sonic hedgehog (Shh), a morphogen molecule implicated in embryonic tissue patterning, displays inductive, proliferative, neurotrophic and neuroprotective activities on various neural cells. Shh might exert its biological functions through binding to patched (Ptc) associated with smoothened (Smo), le...

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Published in:The European journal of neuroscience 1999-09, Vol.11 (9), p.3199-3214
Main Authors: Traiffort, Elisabeth, Charytoniuk, Dorota, Watroba, Laurent, Faure, Hélène, Sales, Nicole, Ruat, Martial
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creator Traiffort, Elisabeth
Charytoniuk, Dorota
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description Sonic hedgehog (Shh), a morphogen molecule implicated in embryonic tissue patterning, displays inductive, proliferative, neurotrophic and neuroprotective activities on various neural cells. Shh might exert its biological functions through binding to patched (Ptc) associated with smoothened (Smo), leading to downstream activation of target genes such as the transcription factor Gli1. We have performed a detailed localization of cells expressing transcripts of Shh, Ptc, Smo and Gli1 in brain and spinal cord of the adult rat as well as in the developing cerebellum. In the adult, Shh‐positive cells were mainly observed in forebrain structures, in the Purkinje cells of the cerebellum and in motor neurons. Ptc‐positive cells were frequently observed in brain areas devoid of any Shh transcripts, except in the median eminence or the facial nucleus, suggesting local Shh signalling. Interestingly, Smo transcripts were predominantly present within circumventricular organs, in granular cells of the dentate gyrus and in neurons of the reticular thalamic nucleus. The presence of Shh, Ptc and Smo transcripts in hypothalamic areas may indicate a role of Shh signalling in the modulation of neuroendocrine functions. The expression pattern of these three genes as well as of Gli1, and their developmental regulation in the cerebellum, suggest a possible role for Hedgehog signalling in the control of various cell populations within the cerebellum, particularly in granule cell proliferation and/or differentiation that might be impaired in proliferative states such as medulloblastomas.
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subjects Animals
Blotting, Northern
Brain cancer
Brain Chemistry
Brain Chemistry - physiology
Brain Mapping
Brain Neoplasms
Brain Neoplasms - physiopathology
Central Nervous System
Central Nervous System - growth & development
Central Nervous System - physiology
Digoxigenin
Factor IX
Factor IX - biosynthesis
Factor IX - genetics
Gene Expression Regulation
Gene Expression Regulation - physiology
Gli1
Hedgehog Proteins
Hybridization
In Situ Hybridization
Life Sciences
Male
medulloblastomas
motor neuron
Mutation
Nervous system
Neurons
Neurons and Cognition
patched
Protein Biosynthesis
Proteins
Proteins - genetics
Rats
Rats, Wistar
RNA
RNA - biosynthesis
RNA - isolation & purification
RNA Probes
RNA, Messenger
RNA, Messenger - biosynthesis
Signal Transduction
Signal Transduction - physiology
smoothened
Spinal Cord
Spinal Cord - metabolism
Trans-Activators
Transcription factors
Tumors
title Discrete localizations of hedgehog signalling components in the developing and adult rat nervous system
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