Complement Receptor 3 (CD11b/CD18) Mediates Type I and Type II Phagocytosis During Nonopsonic and Opsonic Phagocytosis, Respectively

Two types of opsonic phagocytosis have been defined depending on the receptor engaged: FcgammaRs mediate type I phagocytosis of IgG-coated particles; complement receptor 3 (CR3) mediates type II phagocytosis of complement-coated particles. In addition to opsonic phagocytosis, CR3 also mediates nonop...

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Bibliographic Details
Published in:The Journal of immunology (1950) 2002-08, Vol.169 (4), p.2003-2009
Main Authors: Le Cabec, Veronique, Carreno, Sebastien, Moisand, Andre, Bordier, Christine, Maridonneau-Parini, Isabelle
Format: Article
Language:English
Subjects:
Animals
Cellular Biology
CHO Cells
Complement System Proteins
Complement System Proteins - metabolism
Cricetinae
Humans
Immunoglobulin G
Immunoglobulin G - metabolism
Life Sciences
Macrophage-1 Antigen
Macrophage-1 Antigen - metabolism
Microscopy, Electron
Mycobacterium kansasii
Mycobacterium kansasii - immunology
Opsonin Proteins
Opsonin Proteins - immunology
Phagocytosis
Phagocytosis - immunology
Recombinant Proteins
Recombinant Proteins - immunology
Signal Transduction
src-Family Kinases
src-Family Kinases - immunology
Transfection
Zymosan
Zymosan - immunology
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Summary:Two types of opsonic phagocytosis have been defined depending on the receptor engaged: FcgammaRs mediate type I phagocytosis of IgG-coated particles; complement receptor 3 (CR3) mediates type II phagocytosis of complement-coated particles. In addition to opsonic phagocytosis, CR3 also mediates nonopsonic phagocytosis of zymosan (Z) and Mycobacterium kansasii through engagement of distinct sites. Using Chinese hamster ovary cells stably expressing human CR3, we studied CR3-mediated ingestion of nonopsonized particles, Z or M. kansasii, compared with opsonized zymosan (OZ). We show that 1) while OZ sinks into cells, Z is engulfed by pseudopodia as visualized by electron microscopy; 2) in contrast to OZ, nonopsonic phagocytosis of Z and M. kansasii depends on Rac and Cdc42 but not on Rho activity; and 3) CR3-mediated phagocytosis of Z depends on the kinase activity of the Src family tyrosine kinase Hck, while OZ internalization does not. Therefore, CR3 mediates type I phagocytosis under nonopsonic conditions and type II under opsonic conditions. This is the first evidence that a single receptor can mediate both types of phagocytosis depending on the ligand used.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.169.4.2003