Peptide Immobilization on Amine-Terminated Boron-Doped Diamond Surfaces

This paper reports on the formation and characterization of semicarbazide termination on aminated boron-doped diamond (BDD) surfaces, and further preparation of peptide microarray through site-specific α-oxo semicarbazone ligation. Hydrogen-terminated BDD electrodes were first aminated using NH3 pla...

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Bibliographic Details
Published in:Langmuir 2007-04, Vol.23 (8), p.4494-4497
Main Authors: Coffinier, Yannick, Szunerits, Sabine, Jama, Charafeddine, Desmet, Rémi, Melnyk, Oleg, Marcus, Bernadette, Gengembre, Léon, Payen, Edmond, Delabouglise, Didier, Boukherroub, Rabah
Format: Article
Language:English
Subjects:
Amines - chemistry
Boron - chemistry
Chemical Sciences
Chemistry
Chemistry, Physical - methods
Diamond
Electrodes
Exact sciences and technology
General and physical chemistry
Hydrogen - chemistry
Material chemistry
Models, Chemical
Organic chemistry
Peptides - chemistry
Protein Array Analysis
Semicarbazides - chemistry
Spectrometry, Fluorescence
Spectrometry, X-Ray Emission
Surface Properties
Temperature
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Summary:This paper reports on the formation and characterization of semicarbazide termination on aminated boron-doped diamond (BDD) surfaces, and further preparation of peptide microarray through site-specific α-oxo semicarbazone ligation. Hydrogen-terminated BDD electrodes were first aminated using NH3 plasma treatment and then reacted with triphosgene and Fmoc-protected hydrazine to yield a protected semicarbazide termination. Subsequent deprotection and chemical reaction with glyoxylyl peptides led to the covalent immobilization of the peptides on the surface through site-specific ligation. The resulting surfaces were characterized using X-ray photoelectron spectroscopy (XPS) and fluorescence measurements.
ISSN:0743-7463
1520-5827
DOI:10.1021/la063440y