Development of a bipartite method for Fusarium identification based on cellobiohydrolase-C: CAPS and Western blot analysis

The identification of 12 Fusarium strains isolated from diseased hops (Humulus lupulus, L.) was achieved by a strategy based on cellobiohydrolase-C: cleaved amplified polymorphic sequence analysis targeting the gene and the use of an antibody directed against a peptide of the Fusarium graminearum en...

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Bibliographic Details
Published in:FEMS microbiology letters 2002-08, Vol.213 (2), p.245-249
Main Authors: Hatsch, D, Phalip, V, Jeltsch, J.M
Format: Article
Language:English
Subjects:
Antibodies
Biochemistry, Molecular Biology
Biological and medical sciences
Blotting, Western
Cellobiohydrolase
Cellulase
Cellulase - analysis
Cellulase - genetics
Cellulose 1,4-beta-Cellobiosidase
Cleaved amplified polymorphic sequence
DNA, Fungal
DNA, Fungal - analysis
Fundamental and applied biological sciences. Psychology
Fusarium
Fusarium - classification
Fusarium - enzymology
Fusarium - genetics
Fusarium graminearum
Fusarium identification
Gene Amplification
gene targeting
Hops
Humulus lupulus
Immunodetection
Life Sciences
Microbiology
Mycological methods and techniques used in mycology
Mycology
Polymerase Chain Reaction
sequence analysis
Western blotting
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Summary:The identification of 12 Fusarium strains isolated from diseased hops (Humulus lupulus, L.) was achieved by a strategy based on cellobiohydrolase-C: cleaved amplified polymorphic sequence analysis targeting the gene and the use of an antibody directed against a peptide of the Fusarium graminearum enzyme. This strategy is shown to be rapid and reliable for all the Fusarium of our collection: F. avenaceum, F. graminearum, F. sambucinum, F. sporotrichioides, F. tricinctum and F. venenatum.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2002.tb11313.x