Assessment of genetic stability of Brucella melitensis Rev 1 vaccine strain by multiple-locus variable-number tandem repeat analysis

Abstract The assessment of the genetic stability is one of the essential elements to guarantee the biological quality of live anti-bacteria vaccines. Live attenuated Brucella melitensis Rev 1 is the most effective vaccine against brucellosis in small ruminants. Thirty-six B. melitensis Rev 1 vaccine...

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Bibliographic Details
Published in:Vaccine 2007-04, Vol.25 (15), p.2858-2862
Main Authors: García-Yoldi, David, Le Fleche, Philippe, Marín, Clara M, De Miguel, María J, Muñoz, Pilar M, Vergnaud, Gilles, López-Goñi, Ignacio
Format: Article
Language:English
Subjects:
Allergy and Immunology
Animals
Applied microbiology
Bacterial Vaccines
Bacterial Vaccines - genetics
Bacteriology
Biochemistry, Molecular Biology
Biological and medical sciences
Brucella
Brucella - genetics
Brucella - immunology
Brucella melitensis
Brucella melitensis Rev 1
Brucellosis
Brucellosis - virology
Cellular Biology
Cluster Analysis
Fundamental and applied biological sciences. Psychology
Genetic stability
Genomic Instability
Genotype
Humans
Life Sciences
Microbiology
Minisatellite Repeats
Miscellaneous
MLVA
Ruminantia
Ruminants
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
VNTR
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Summary:Abstract The assessment of the genetic stability is one of the essential elements to guarantee the biological quality of live anti-bacteria vaccines. Live attenuated Brucella melitensis Rev 1 is the most effective vaccine against brucellosis in small ruminants. Thirty-six B. melitensis Rev 1 vaccine strains isolated from human or animal sources from different geographic regions, from different commercial batches or laboratory collections were typed by the multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA) recently described for Brucella spp. Our results demonstrated that B. melitensis Rev 1 group as assayed by MLVA is genetically very homogeneous. We believe that MLVA methodology could be an essential assay to guarantee the quality and stability of live anti-bacterial vaccines being produced worldwide and can be included as in vitro control.
ISSN:0264-410X
1873-2518
0264-410X
DOI:10.1016/j.vaccine.2006.09.063