Flagellar length depends on LdARL-3A GTP/GDP unaltered cycling in Leishmania amazonensis

We have shown previously that expression of the GTP-blocked form of the small G protein LdARL-3A/Q70L led to a marked shortening of Leishmania promastigotes flagella. In contrast, there was no effect with the T30N mutant, thought to represent the GDP-blocked form. However, recent data, obtained with...

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Bibliographic Details
Published in:Molecular and biochemical parasitology 2008, Vol.157 (1), p.83-87
Main Authors: Sahin, Annelise, Espiau, Benoît, Marchand, Cécile, Merlin, Gilles
Format: Article
Language:English
Subjects:
Amino Acid Substitution - genetics
Animals
Differentiation
Flagella - genetics
Flagella - physiology
Flagellar length
G protein cycle
Guanosine Diphosphate - metabolism
Guanosine Triphosphate - metabolism
LdARL-3A
Leishmania
Leishmania - cytology
Leishmania - genetics
Leishmania - physiology
Life cycle
Life Sciences
Microbiology and Parasitology
Microscopy, Fluorescence
Microscopy, Phase-Contrast
Protozoan Proteins - genetics
Protozoan Proteins - metabolism
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Summary:We have shown previously that expression of the GTP-blocked form of the small G protein LdARL-3A/Q70L led to a marked shortening of Leishmania promastigotes flagella. In contrast, there was no effect with the T30N mutant, thought to represent the GDP-blocked form. However, recent data, obtained with human ARF-6, a member of the same family of G proteins, revealed that the corresponding mutant T27N was nucleotide-free and that the GDP-blocked form was the T44N mutant. When expressed in Leishmania, the corresponding new mutant, LdARL-3A/T47N, provoked also flagellum shortening. Then, it is the interruption of the cycling of LdARL-3A between a GDP- and a GTP-bound form which leads to the reduction of the flagellar length. This findings change significantly the understanding and the approaches for studying the mode of action and the role of LdARL-3A.
ISSN:0166-6851
1872-9428
DOI:10.1016/j.molbiopara.2007.08.003